Genetic Insights into COVID-19 Severity: The Role of TLR3 and IRF7 Polymorphisms in Egyptian patients | ||
| Journal of Advanced Pharmacy Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 October 2025 | ||
| Document Type: Research Article | ||
| DOI: 10.21608/aprh.2025.411977.1333 | ||
| Authors | ||
| Doaa Mohamed Abd-Allah1; Shahenda Mahgoub* 2; Hanaa Beskales Atya3; Mohamed Elbadry4; Mohamed El-Kassass4; Sahar A. Ali5 | ||
| 1Pharmacovigilance Department, Directorate of Health Affairs, Ministry of Health. | ||
| 2Biochemistry & Molecular Biology Department, Faculty of Pharmacy, Helwan University, Helwan, Cairo, Egypt | ||
| 3Biochemistry and Molecular Biology Department, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo, Egypt. | ||
| 4Endemic Medicine Department, Faculty of Medicine, Helwan University , Cairo, Egypt. | ||
| 5Professor at Department of Biochemistry and Molecular Biology Faculty of Pharmacy,Helwan University | ||
| Abstract | ||
| Objectives: This study was conducted to explore the impact of TLR3 (rs3775296) and IRF7 (rs373394984) gene polymorphisms on the severity of COVID-19 in Egyptian patients. Methods: The present study included 175 COVID-19 patients, 87 with severe COVID-19 symptoms and 88 who were asymptomatic or with mild symptoms. Blood samples were collected from all patients for DNA extraction. DNA Genotyping assay was performed for detecting specific SNP (rs3775296) and (rs373394984) in the purified genomic DNA samples. Results: Although the two studied SNPs (rs3775296 and rs373394984) did not show a statistically significant difference between severe and mild COVID-19 cases, they may still have a protective effect. Our results indicate that both SNPs are associated with reduced severity, as the odds ratios are less than 1. Individuals carrying the mutant A allele have a 0.3 times lower likelihood of developing severe symptoms for rs373394984 and 0.5 times lower for rs3775296 compared to non-carriers. Furthermore, the mutant A allele of rs373394984 was predominant in cases which were associated with other co-morbidities. Conclusion: The studied SNPs (rs3775296 and rs373394984) may have a protective effect. This effect may suggest that these SNPs are involved in mechanisms that reduce severity of the disease. These findings highlight the potential role of genetic factors in COVID-19 severity and may contribute to a better risk assessment and targeted interventions in affected populations. | ||
| Keywords | ||
| COVID-19; SNP; IRF7; TLR3; comorbidities | ||
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