Developmental Asymmetry of Ovary in Rhode Island Red Chicken Embryos: A Histological, Histochemical and Immunohistochemical Study | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 01 October 2025 PDF (1.52 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.406000.2980 | ||
| Authors | ||
| usama Kamal Moawad* 1; Abdel-Razik Hashem Abdel-Razik2; Tasneem Ashraf Fathy1; Ranada Mohamed Hassan1 | ||
| 1Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt | ||
| 2Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 6 62511, Egypt | ||
| Abstract | ||
| The present study aimed to investigate the developmental asymmetry of the left ovary of Rhode Island Red (RIR) chicken embryos using histological, histochemical, and immunohistochemical techniques. Embryos at the following stages were examined at days: 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, 19 & 21, with three embryos per stage. Serial 3-5 μm sections of whole embryos and ovaries were prepared and stained using Hematoxylin and Eosin (H&E), Periodic Acid-Schiff (PAS), and Masson's Trichrome stain, along with immunohistochemical staining. The results revealed significant bilateral asymmetry, with the left ovary exhibiting a more developed cortex and medulla at all stages. The primitive left ovarian cortex was composed of germinal epithelium, germ cells, and numerous somatic cells. As development progressed, the left ovary enlarged, with an increase in the size of secondary sex cords. The medulla of the left ovary contained isolated germ cells, numerous lacunar channels, interstitial cells, blood vessels, and nerves. Immunohistochemical analysis at 14 and 18 days of embryonic development revealed estrogen receptor-positive cells in both the ovarian surface epithelium and the germ cells. Progesterone receptor-positive cells, however, were only identified in the germ cells and ovarian surface epithelium at day 18. | ||
| Keywords | ||
| Embryonic development; Germ cells; Immunohistochemistry; Ovarian asymmetry; Rhode Island Red chicken | ||
|
Statistics Article View: 59 PDF Download: 45 |
||
