Determination of Cefixime in Pharmaceutical Dosage Forms and Biological Samples: Current Analytical Strategies | ||
| AlSalam International Journal of Pharmacy | ||
| Volume 2, Issue 1, September 2025, Pages 33-43 PDF (420.15 K) | ||
| Document Type: Scientific review | ||
| DOI: 10.21608/aijp.2025.410776.1006 | ||
| Authors | ||
| Eman Salem; Amr Tayel* ; Mohamed Abdelhamied; Mokhtar Mabrouk | ||
| Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Alsalam University, Tanta, Egypt | ||
| Abstract | ||
| Cefixime is an orally administered, third-generation cephalosporin antibiotic belonging to the β-lactam class, widely used for its broad-spectrum activity against numerous bacterial pathogens. Its mechanism of action involves binding to bacterial penicillin-binding proteins (PBPs), thereby disrupting the final steps of peptidoglycan cross-linking in cell wall synthesis. This inhibition weakens the bacterial cell wall, leading to lysis and death. Cefixime is active against gram-positive bacteria such as Streptococcus pneumoniae and gram-negative pathogens, including Escherichia coli, Klebsiella pneumoniae, Haemophilus influenzae, and Neisseria gonorrhoeae. Clinically, it is employed in treating respiratory and urinary tract infections, otitis media, and uncomplicated gonorrhea. Its oral availability, favorable safety profile, and once- or twice-daily dosing make it a preferred choice for outpatient therapy. This review consolidates current analytical methods for detecting and quantifying Cefixime in raw drug materials, pharmaceutical formulations, and biological samples. Bioanalytical techniques are crucial for accurately assessing drug concentration and metabolism, essential for pharmacokinetic and therapeutic monitoring studies. Various laboratory-based techniques have been utilized, including spectrophotometry, spectrofluorimetry, high-performance liquid chromatography (HPLC), high-performance thin-layer chromatography (HPTLC), and ultra-performance liquid chromatography (UPLC). The review aims to provide a comprehensive overview of these methods, facilitating advancements in drug analysis, formulation development, and clinical applications of Cefixime. | ||
| Keywords | ||
| Cefixime; Cephalosporin antibiotic; Analytical methodologies | ||
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