Molecular detection of aminoglycoside modifying enzyme aphA6 in Acinetobacter baumannii clinical isolates | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 02 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.417294.3147 | ||
| Authors | ||
| Elaf Akram Fadhil* 1; Rafal Khaleel Farhan2 | ||
| 1Department of Biology, College of Science/ University of Tikrit, Tiktit, Iraq | ||
| 2Department of Medical Microbiology, College of Medicine, Tikrit University, Tikrit, Iraq | ||
| Abstract | ||
| Background: This bacterium is one of the common pathogens causing clinical illness in a variety of clinical cases, including urinary tract infections, gut infections, as well as wound and burn sites. This study was designed to isolate Acinetobacter baumannii from clinical samples and asses its resistance to aminoglycosides phenotypically and molecularly. Methods: The study involved sampling 230 clinical specimens to identify Acinetobacter baumannii from January 2025 until May 2025 at Tikrit Teaching Hospital, Iraq. All specimens underwent routine culture and biochemical identification in addition to confirmation via the DL microbiology identification kit, which involves both confirmation and an antibiotic susceptibility test to determine the aminoglycoside-resistant pattern. Plasmid extraction was performed on all isolates, and PCR amplification of the aphA6-aphA6 gene was performed on all isolates. Results: Our data revealed a female-to-male ratio of 60:40, with mean ages of 47 years for males and 35 years for females. Out of 230 clinical isolates, Acinetobacter baumannii represented 13.91%, and the aminoglycoside-resistant group specifically for gentamicin, amikacin, and tobramycin, was 53%, 59%, and 68%. Genetic detection of aphA6 was found in nearly 47% of all Acinetobacter baumannii isolates. Conclusion: In conclusion, Acinetobacter baumannii isolated from clinical cases illustrated a multidrug-resistantnt pattern, especiallyly to the aminoglycoside group, and tested positive due to the presence of the aminoglycoside-modifying enzyme aphA6, which greatly increases the ability of the bacteria to resist antibiotics. | ||
| Keywords | ||
| Aminoglycoside; A. baumanni; Amikacin; Gentamycin | ||
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