Nano-Pichia guilliermondii as a Novel Dietary Supplement in Oreochromis niloticus: Impacts on Growth, Immune-Antioxidant Functions, Intestinal Morphometrics, and Resistance to Aeromonas hydrophila Challenge | ||
| Zagazig Veterinary Journal | ||
| Volume 53, Issue 3, September 2025, Pages 280-299 PDF (473.95 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zvjz.2025.380496.1283 | ||
| Authors | ||
| Mohamed Hasanin1; Abdelhakim Elmor1; Amr R. EL-Khattib1; Mohamed Metywally Mohamed2; Sameh H. Ismail3; Rowaida Kamhawey* 1 | ||
| 1Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt | ||
| 2Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt | ||
| 3Faculty of Nanotechnology for Postgraduate Studies, Cairo University, Sheikh Zayed Branch Campus, Sheikh Zayed City, Giza, PO Box 12588, Egypt | ||
| Abstract | ||
| Recently, nanoparticles have been used in aquafeed, benefiting fish health and growth and promoting their sustainable use in aquaculture. The present investigation examined the potential advantages of feeding Nile tilapia (Oreochromis niloticus) with nano-Pichia guilliermondii (NPG) on their growth, antioxidant-immune metrics, digestive processes, and tolerance to the Aeromonas hydrophila challenge. For 70 days, 135 fish (body weight =33.36±0.31 g) were allocated equally into three groups of 45 fish each, with 15 fish per replicate. Basal diets fortified with 0%, 0.1 %, and 0.2% NPG /kg were given to the CONT, NPG 0.1, and NPG 0.2 groups, respectively. After the feeding experiment, each group was challenged with 0.1 mL (1.5×106) of A. hydrophila, and the fish survival was tracked for 15 days post challenge. The results demonstrated that dietary supplementation of NPG 0.2 followed by NPG 0.1 decreased serum glucose levels while increased growth metrics, growth hormone levels, and digestive enzymes’ (lipase and amylase) activities as compared with the control group. Additionally, NPG-supplemented diets improved antioxidants (superoxide dismutase, catalase, and total antioxidant capacity) and immunological (lysozyme, complement 3, and phagocytic) characteristics. NPG fortification maintained the normal intestinal histology and produced no pathological abnormalities, and the NPG 0.2 diet considerably enhanced the width of the intestinal villi. The fish fed NPG were more resilient to the A. hydrophila challenge, as evidenced by the increased survival rates of the NPG 0.2 group (83.3%) and NPG 0.1 group (75%), compared to the CONT group (66.7%). Dietary NPG, particularly at the 0.2 % level, may be a viable feed additive for Nile tilapia’s diets to enhance their growth, well-being, and ability to withstand bacterial pathogens. | ||
| Keywords | ||
| Oreochromis niloticus; yeast nanoparticles; Feed additive; A. hydrophila; Immune responses | ||
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