Utilization of Green Synthesized Chitosan Gold Nanoparticles for Oral Cancer Treatment Using Laser Flow Cytometry: In-Vitro Study
Alaaeldin, S., Hamdy, O., El-tayeb, E., Zaky, A., Deif, H., Faid, A. (2025). Utilization of Green Synthesized Chitosan Gold Nanoparticles for Oral Cancer Treatment Using Laser Flow Cytometry: In-Vitro Study. EKB Journal Management System, (), -. doi: 10.21608/ejchem.2025.408225.12126
Sara Alaaeldin; Omnia Hamdy; El-Sayed El-tayeb; Ahmed Abbas Zaky; Heba NM Deif; Amna Faid. "Utilization of Green Synthesized Chitosan Gold Nanoparticles for Oral Cancer Treatment Using Laser Flow Cytometry: In-Vitro Study". EKB Journal Management System, , , 2025, -. doi: 10.21608/ejchem.2025.408225.12126
Alaaeldin, S., Hamdy, O., El-tayeb, E., Zaky, A., Deif, H., Faid, A. (2025). 'Utilization of Green Synthesized Chitosan Gold Nanoparticles for Oral Cancer Treatment Using Laser Flow Cytometry: In-Vitro Study', EKB Journal Management System, (), pp. -. doi: 10.21608/ejchem.2025.408225.12126
Alaaeldin, S., Hamdy, O., El-tayeb, E., Zaky, A., Deif, H., Faid, A. Utilization of Green Synthesized Chitosan Gold Nanoparticles for Oral Cancer Treatment Using Laser Flow Cytometry: In-Vitro Study. EKB Journal Management System, 2025; (): -. doi: 10.21608/ejchem.2025.408225.12126

Utilization of Green Synthesized Chitosan Gold Nanoparticles for Oral Cancer Treatment Using Laser Flow Cytometry: In-Vitro Study

Egyptian Journal of Chemistry
Articles in Press, Accepted Manuscript, Available Online from 02 October 2025
Document Type: Original Article
DOI: 10.21608/ejchem.2025.408225.12126
Authors
Sara Alaaeldin1; Omnia Hamdy* 2; El-Sayed El-tayeb3; Ahmed Abbas Zaky1; Heba NM Deif4; Amna Faid5
1Department of Medical Applications of Laser, National Institute of Laser Enhanced Sciences (NILES), Cairo University
2Cairo University
3National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza, Egypt
4Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
5Department of laser sciences and interactions, National Institute of Laser Enhanced Sciences (NILES), Cairo University
Abstract
This study reports the eco-friendly synthesis, comprehensive characterization, and in vitro cytotoxic evaluation of chitosan-reduced gold nanoparticles (Cs@AuNPs). Cs@AuNPs were synthesized by reducing chloroauric acid using chitosan as both a reducing and stabilizing agent, yielding a stable ruby-red colloidal solution. The formation of nanoparticles was confirmed by UV-Visible spectroscopy, with a surface plasmon resonance peak observed at 522 nm. Transmission electron microscopy revealed spherical nanoparticles with a size distribution of 14 ± 2 nm, while zeta potential measurements indicated high colloidal stability (+28 mV). FTIR analysis confirmed the involvement of chitosan’s amino and hydroxyl functional groups in the reduction and stabilization processes. The cytotoxic potential of Cs@AuNPs was assessed using the sulforhodamine B (SRB) assay on oral epithelial cells (OEC), revealing dose-dependent effects with acceptable cell viability at concentrations up to 100 μg/mL. Further analysis using Annexin V-FITC/PI flow cytometry demonstrated that Cs@AuNPs induced apoptosis in a concentration-dependent manner, with early and late apoptotic populations clearly distinguishable. These findings highlight the biocompatibility and potential therapeutic application of Cs@AuNPs, suggesting their suitability for further development in cancer diagnostics and targeted delivery systems.
Keywords
Chitosan; Flow Cytometry; Gold Nanoparticles; Green Synthesis; Oral Cancer; Oral Epithelial Cells
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