Effect of cytokinins on Photosynthetic pigments and protein content of micropropagated Mentha viridis L. and Moringa oleifera Lamunder white and violet visible light emitting diodes (LEDs) | ||
| Mansoura Journal of Biology | ||
| Volume 59, Issue 6, December 2022, Pages 62-73 PDF (919.81 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2022.456548 | ||
| Authors | ||
| Lina; M. Abu-Ziada* 1; Samia, A. Haroun1; Rasha, M. E. Gamel1; Mohamed, A. Abbas1; Heba Y. El-Banna2 | ||
| 1Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt | ||
| 2Vegetable and Floriculture Department, Faculty of Agriculture, Mansoura University | ||
| Abstract | ||
| The present study was carried out at the tissue culture lab and experimental station of the Vegetable and Floriculture Department, faculty of Agriculture, Mansoura University. Propagation of two medicinal plants grown for 30 days in a protocol wheremature seeds of Moringa oleifera was used invitro culture, while for Mentha viridis the explant material of nodal segment (about 1-1.5 cm) was used. Two types of cytokinins: 6-benzylaminopurin (BAP), and thidiazuron (TDZ) singly at three different concentrations (0, 1, or 2 mgL-1) were used as well as light-emitting diode systems (LEDs) (white as control and violet; the combination of red and blue; 1:1). The results cleared that a general increment in chlorophyll a, chlorophyll b, carotenoids and total pigments by all the used treatments either under white LEDs or violet LEDs illumination in case of the two used plants, as compared to control (free hormone medium). So, under violet LEDs illumination, the maximal value of total pigments (25.717 mg/g dry weight) in Mentha was recorded in case of MS medium supplemented with 2 mgL-1 TDZ and control medium recorded the least total pigments values (13.587 mg/g, respectively) under white LEDs illumination. In Moringa maximum total photosynthetic pigments were 29.967 mg/g, in MS medium with 2 mgL-1BAP under violet LEDs and the minimum value (18.359 mg/g) was in MS medium with 1 mgL-1 TDZ under white light conditions; as there wasn’t any shoots or leaves at all.The maximal values of total protein content in micropropagated Mentha and Moringa were in case of MS nutrient medium supplemented with 2 mgL-1 TDZ under violet LEDs illumination (46.7007 and 52.164 mg/g fresh weight), respectively. On the other hand, the minimal value of protein content in Mentha was in control (free hormone medium) MS medium under white LEDs (21.933 mg/g fresh weight) and in Moringa was in MS medium fortified with 1 mgL-1 BAP under white LEDs (20.544 mg/g fresh weight). | ||
| Keywords | ||
| Micropropagation; light emitting diodes (LEDs); photosynthetic pigments; benzyl 6- amino-purine; thidiazuron; Mentha viridis; Moringa oleifera | ||
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