Genotypic study of different phylogroup Escherichia coli causes of pediatric diarrhea in Al-Najaf Province, Iraq | ||
Microbes and Infectious Diseases | ||
Articles in Press, Accepted Manuscript, Available Online from 03 October 2025 | ||
Document Type: Original Article | ||
DOI: 10.21608/mid.2025.409635.3067 | ||
Authors | ||
Ban Amer Helal; zahraa Yosif Motaweq* | ||
Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq | ||
Abstract | ||
Background: Conventionally, in Escherichia coli, phylogenetic groups A and B1 are associated with commensal strains, while B2 and D are associated with extraintestinal strains. The aim of this study was to evaluate diarrheagenic E. coli (DEC) phylogeny and its association with biofilm formation. The result indicated that 72 E. coli were isolated from 120 diarrheal stools. Most of these isolates displayed a moderate ability to form a biofilm (bacterial adherence) through registered moderate adherence reaching between 0.120 and 0.254. The findings of the CRA techniques, which involved growing all bacterial isolates on Congo red agar to identify biofilm development, revealed that 79.1% of isolates of E. coli produced robust and moderate biofilms, whereas 20.9% produced weak biofilms. E. coli strains are genetically diverse, and fall into four main phylogenetic groups: A, B1, B2, and D. The result of PCR-based phylotyping showed that the most distributed phylogenetic group of E. coli was Phylogenetic group D which comprised 13/24 (54.16%), the high prevalence of phylogroup D was found among the isolates obtained from hospital-acquired infection rather than community-acquired infection, then followed by Phylogenetic group B2 strains 7/24 (29.16%), Phylogenetic group A that comprised 3/24 (12.5%) and finally the Phylogenetic group B1 was 1/24 (4.16%). Phylogenetic groups and biofilm formation of 72 E. coli isolates (DEC from children with diarrhea): isolated from Iraqi children, there was a tendency for a higher proportion of persistent diarrhea (≥14 days) among severe groups (B2 and D). | ||
Keywords | ||
Pediatric diarrhae; Biofilm Formation; Escherichia coli; Phylogroup | ||
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