Reveal by genotyping and phenotyping methods of some Metallo- Beta Lactamases genes among environmental Klebsiella pneumoniae isolates | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 04 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.400106.2980 | ||
| Authors | ||
| Jinan Mohammed Hasan* ; Enass Ghassan Sweedan; Dimah Nazar Faraj | ||
| Biology department, College of Science, University of Baghdad | ||
| Abstract | ||
| Background: Since carbapenems are currently the preferred treatment for severe infections brought on by multidrug-resistant bacteria which can create Extended-Spectrum β-Lactamases (ESBLs), it is extremely concerning that Gram-negative bacteria are becoming resistant to carbapenem. It has been demonstrated that Klebsiella pneumoniae produces a beta-lactamase that hydrolyses the β-lactam ring in antibiotics, making it one of the few bacteria which are currently exhibiting a high value of resistance because of changing in the organism's core genome. Methods: For the current study, 50 samples were gathered from different water sources, and based on morphological and biochemical testing, 10 isolates were determined to be K. pneumoniae. According to Kirby and Bauer's disc diffusion test, all of the isolates under investigation exhibited resistance to Ampicillin, Cefitrixone, Amoxicillin, Ticarcillin+Clavulanic acid, Ceftazidime, and Ticarcillin. Every specimen that had the highest percentage of resistant isolates was 100% effective against the antibiotics being studied. E-test strips were used to detect the isolates' Minimum Inhibitory Concentrations (MICs) value. Results: Upon using Combined Disc Test (CDT) to detect Metallo- beta lactamases, it was found that 8.3% of isolates were MBLs producers. Nevertheless, PCR technique exposed those three isolates only harbored IMP-1 gene and no one was had NDM-1 gene. Conclusions: Current study found relationship between IMP-1 and resistant to antibiotics, when P≤0.05. For that K. pneumoniae isolated from water began resist to carbapenems antibiotics by horizontal way or by plasmid from clinical isolates to environmental isolates. | ||
| Keywords | ||
| beta- lactamase; MDR resistance bacteria; IMP-1 gene; NDM-1 gene; Carbapenem | ||
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