Comparison Between Egg Yolk and Soybean Lecithin in a Tris-Based Extender on Ram Sperm Quality | ||
| Assiut Journal of Agricultural Sciences | ||
| Volume 56, Issue 4, October 2025, Pages 36-55 PDF (1.11 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajas.2025.414976.1529 | ||
| Authors | ||
| Motlaq H. M. Al-hajri1; Taymour M. EL-sherry2; Hassan A. Daghash3; Gamal B. Mahmoud* 3; Haitham A. Mofadel2 | ||
| 1Public Authority for Agriculture and Fisheries, Kuwait. | ||
| 2Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt. | ||
| 3Department of Animal Production, Faculty of Agriculture, Assiut University, Assiut, Egypt. | ||
| Abstract | ||
| This study aimed to compare between the addition of egg yolk (EG; 20%) and soybean lecithin (SL; 1%) to a Tris-based extender on total sperm motility, viability and reheotaxis parameters, including Positive Rheotaxis (PR%); Straight Line Velocity (VSL, µm/s); Curvilinear Velocity (VCL, µm/s); Linearity (LIN = VSL/VCL) Average Path Velocity (VAP, µm/s) and Beat Cross Frequency (BCF, Hz) in semen. Sperm kinetics and rheotaxis parameters were investigated using computer-assisted sperm analysis (CASA) with regulated flow velocity. Semen was collected using artificial vagina once weekly from 6 fertile rams at 9:00 a.m. during the late spring and autumn. Samples of pooled semen were diluted in one of the two fractions, with the primary extender composed of tris-based extender, with 20% egg yolk for the control group and the percentages of soybean lecithin (1%) for the treatment group. Results revealed that there were no significant differences in total sperm motility, viability and PR % and VAP between the two extender groups. Also, there were significantly higher VCL and BCF in the soybean lecithin extender group compared to the egg yolk extender group. In the contrast there were significantly higher in VSL and LIN in the egg yolk extender group compared to the soybean lecithin group. In conclusion, the extenders were supplemented with 20% egg yolk, and 1.0% soybean lecithin had relatively similar effects on total sperm motility, viability and reheotaxis parameters. We concluded that a commercially available soybean lecithin (1%) was an alternative to an egg yolk-based extender in preserving motility, viability and reheotaxis parameters of semen. | ||
| Keywords | ||
| Egg yolk extender; Ram semen; Soybean lecithin | ||
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