Impact of Moringa Leaf Extract and Soybean Powder on the Growth rate of Bifidobacterium animalis subsp. Lactis | ||
| Assiut Journal of Agricultural Sciences | ||
| Volume 56, Issue 4, October 2025, Pages 56-68 PDF (1.04 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajas.2025.395109.1503 | ||
| Authors | ||
| Fatma M. Ibrahim1; Asmaa M. Abdel-Rahman2; Aly I. Hassan1; Dalia G. Kamel* 1 | ||
| 1Dairy Science Department, Faculty of Agriculture, Assiut University, Assiut 71515, Egypt | ||
| 2Food Science and Technology Department, Faculty of Agriculture, Assiut University, Assiut 71515, Egypt | ||
| Abstract | ||
| Natural prebiotic compounds could support the growth of probiotic bacteria and contribute to the balance of the intestinal microbiome effectively. The objectives of this study were to evaluate the moringa leaf extract (MLE) and soybean powder (SP) as natural prebiotic substances, added by different concentrations of each (0.5, 1.0, and 1.5% in MLE; 0.1, 0.3, and 0.5% in SP) on the viability of probiotic bacteria (Bifidobacterium animalis subsp. lactis) and acid production in sterilized milk. MLE and SP were sterilized and then were added to pasteurized buffalo skim milk (0.1% fat, 4.31% protein, 9.08% milk SNF,4.1% lactose, and 0.68% salt) after cooled to 40–42°C, Bifidobacterium animalis subsp. lactis (Bif. lactis) was inoculated (6.73 Log10 CFU/ml). Cell population and lactic acid production were determined during 24h. The results indicated that using 1.5% MLE as a prebiotic increased cell population to its maximum level (10.48 log CFU/ml) after 20 hours, while titratable acidity (TA%) recorded its maximum level (0.44± 0.01) after 24 hours of incubation. On the other hand, using 0.5% of SP after 24 hours of incubation recorded the maximum cell population (10.29 ± 0.02 log CFU/ml), and TA% recorded its highest level (0.44 ± 0.01). The results showed a direct relationship between the growth rate of Bif. lactis and rate of increase in the percentage of acidity. Moreover, the viability of Bif. lactis during incubation was enhanced significantly (p <0.05) by the inclusion of MLE and SP. | ||
| Keywords | ||
| Bifidobacterium animalis subsp. lactis; Growth curve; Moringa leaf extract; Soybean powder | ||
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