Isolation and Identification of Phenylalanine Ammonia Lyase (PAL) Enzyme–Producing Microorganisms in Assiut Governorate, Egypt | ||
| Assiut Journal of Agricultural Sciences | ||
| Volume 56, Issue 4, October 2025, Pages 106-119 PDF (1.38 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajas.2025.402982.1514 | ||
| Authors | ||
| Safaa A. Limam1; Abdel-Naser A. Zohri2; Shymaa M. Abdelkhalek* 1; Rania M. Hamdy1 | ||
| 1Dept. of Food Science and Technology, College of Agric., Assiut University, Assiut, Egypt. | ||
| 2Dept. of Botany and Microbiology, College of Science, Assiut University, Assiut, Egypt. | ||
| Abstract | ||
| This study aimed to isolate microorganisms producing phenylalanine ammonia-lyase (PAL) from various locations in Assiut Governorate, Egypt. The results demonstrated that 14 fungal isolates gave positive results for PAL production during primary screening. These isolates were belonging to Aspergillus niger (2 isolates), A. oryzae (1), Fusarium proliferatum (1), Fusarium solani (3), A. flavus (2), A. fumigatus (1), Cladosporium cladosporioides (1), Geotrichum candidum (1), Fusarium oxysporum (1), and A. terreus (1). Additionally, four bacterial isolates coded as YA2, YE2, YB1 and YC1 tested positive for PAL production, while no yeast isolates were PAL-positive. Results also showed that Fusarium solani NO.1 was the highest PAL producer among the tested fungal isolates (the enzyme activity was 0.224 U/min/ml of cell suspension). Moreover, YA2 isolate was the highest bacterial enzyme producer with volume activity of 0.112 U/min/ml of cell suspension. These results demonstrate the diversity of PAL-producing microorganisms, which can be used in industrial enzyme synthesis. | ||
| Keywords | ||
| Bacteria; Fungi; Isolation; Phenylalanine ammonia lyase; Yeast | ||
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