Overcoming multi-drug resistance of genetically characterized Escherichia coli using myco-synthesized selenium nanoparticles with antioxidant activity | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 06 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.424242.3225 | ||
| Authors | ||
| Mohammed A. Kamel1; Ahmed Elsayed Khatab Al ghbashy1; Mohamed Sharaf1; Alsayed E. Mekky* 2 | ||
| 1Department of Biochemistry, Faculty of Agriculture, AL-Azhar University, Nasr City 11651, Cairo, Egypt | ||
| 2Department of Botany and Microbiology , Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt. | ||
| Abstract | ||
| Background: Skin infections caused by resistant Escherichia. coli, particularly in weakened wounds, can be severe and challenging to overcome because of the bacteria's modes of antibiotic resistance. Doctors may assess E. coli isolates' susceptibility to various antibiotics using disk diffusion techniques in order to identify the best course for overcoming. Because bacteria have not developed defenses against green manufactured nanoparticles, which frequently have unique modes of action that circumvent established resistance routes, nanotechnology holds enormous potential in the fight against resistant bacteria. This work aimed to combat MRD E. coli using Selenium nanoparticles produced by certain isolated fungus from the soil. Methods: Bacteria were isolated from wounds from patients in El-Hussein Hospital and tested for their sensitivity using 13 different antibiotics. Soil samples were collected from El-Sharkia and screened for production of Se-NPs. The pathogenic bacteria and soil fungi were characterized using molecular identification. Gas chromatography-mass spectrometry (GC-MS) was done for the fungal filtrate. The produced Se-NPs were characterized using suitable instruments including UV-Vis spectroscopy, Electron microscopy, Fourier-Transform Infrared spectroscopy, X-ray diffraction. The produced Se-NPs was tested versus MDR bacteria using agar diffusion technique. Antioxidant levels of the myco-produced Se-NPs were examined relative to suitable norm Results: MDR E. coli was isolated and identified with a number of PQ451703. While, Aspergillus terreus was isolated from soil and identified with a number PV082229 on Gene Bank. The filtrate of fungus contained 11 various volatile compounds where Cinnamaldehyde, (E)-; and Benzenemethanol, à-methyl-à-propyl- were the prevalent compounds. A. terreus successfully produced Se-NPs which characterized by various techniques and had antibacterial action versus MDR E. coli with inhibition zone of 24.25± 1.2 mm. and MIC level at 125± 1.6 µg/ml. Se-NPs had a good antioxidant potential with IC50= 34.92±2.2 µg/ml Conclusion: A. terreus PV082229.1, could produce Se-NPs with a promising antibacterial activity versus MDR E. coli PQ451703. Future research is needed to confirm the impact of Se-NPs versus resistant bacteria through animals' studies. | ||
| Keywords | ||
| pathogenic; bacteria; antioxidant; Nanoparticles | ||
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