Isolation and Molecular Characterization of Mycoplasma gallisepticum from Domestic Poultry in Sanliurfa, Turkey | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 06 October 2025 PDF (954.95 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.396895.2951 | ||
| Authors | ||
| Ayfer Güllü Yücetepe* ; Oktay Keskin | ||
| Harran University Faculty of Veterinary Medicine Department of Microbiology | ||
| Abstract | ||
| The aim of this study was to investigate the isolation of Mycoplasma gallisepticum from domestic poultry and the molecular characterization of the isolated strains. Tracheal swabs and tissue samples from 120 domestic poultry were used to isolate the pathogen. Seven M. gallisepticum were isolated from 120 samples (5.8%). Thirty of 62 (48.3%) chickens and 35 of 58 (60.3%) turkeys tested positive by PCR. PCR amplicons detected between 750-800 bp from the target gene regions of mgc2F and mgc2R and digested with the restriction enzymes AluI, HaeII and HinfI yielded two different PCR-RFLP types. Phylogenetic analysis of the mgc2 gene region formed two groups. In addition, a deletion of 63 nucleotides in the base sequences of the mgc2 gene was only detected in M. gallisepticum 6/85 and the isolates of group 1. Protein analysis of the isolates by SDS-PAGE clearly revealed bands with molecular weights of 120,100,70, 64, 67,56, 53,45,43 and 26 kDa in all isolates. In the WB analysis, antibodies against M. gallisepticum-specific proteins with molecular weights of 200, 120,100, 98,67, 64,40,35,26 kDa were detected. We concluded that further work on different genes of M. gallisepticum to distinguish more specifically between field isolates is needed based on the available data. In addition, naturally infected flocks can be distinguished from vaccinated flocks, which is a very effective method to control and monitor infection. | ||
| Keywords | ||
| CRD; M. gallisepticum; poultry mycoplasmosis; mgc2 gene; western blot | ||
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