PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF INTRAUTERINE PATHOGENIC ESCHERICHIA COLI WITH MULTI-DRUG RESISTANCE ISOLATED FROM COW UTERI WITH ENDOMETRITIS | ||
| Assiut Veterinary Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 07 October 2025 PDF (719.81 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2025.375252.1661 | ||
| Authors | ||
| HUSSIEN, A.M. ABDUL ALIME1; AHMED, A.A. MAAROUF2; MOHAMED REFEAY OSHBA3; HAYAT FAYED* 4; ASHRAF, A. ABD EL TAWAB1 | ||
| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt. | ||
| 2Department of Bacteriology, Immunology & Mycology, Animal Health Research Institute "Benha branch" (ARC). | ||
| 3Diagnostic Imaging and Endoscopy Unit (DIEU), Animal Reproduction Research Institute (ARRI), Agriculture Research Centre (ARC). | ||
| 4Department of Animal Medicine (Internal Medicine), Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt. ORCID number: 0009-0007-3251-0602 | ||
| Abstract | ||
| Endometritis is a common postpartum disorder affecting cows, leading to financial losses. E. coli is among the most prominent clinically significant pathogens responsible for severe cases of bovine endometritis. 110 uterine swab samples from cows from several veterinary clinics at Kaliobia Governorate, Egypt (75 cows had subclinical endometritis and 35 cows had clinical endometritis) were used in order to isolate and identify pathogenic E. coli and to assess their susceptibility to antibiotics, in addition to their genotypic and phenotypic description of virulence and antimicrobial resistance genes. Our findings provided that endometrial E. coli was isolated from 54 examined uterine samples (49.1%). The identified E. coli was sensitive to norfloxacin, followed by gentamycin, ciprofloxacin, and co-trimoxazole, whereas remarkably resistant to oxacillin, ampicillin, tetracycline, cefotaxime, cephapirin, and streptomycin. All 54 isolated E.coli had Congo Red binding activity, and also all the 45 isolates showed phenotypic evidence of biofilm development. PCR results revealed that fimH was present in all five studied E. coli strains, and three of them contained papC virulence genes. Antimicrobial resistant genes blaTEM , sul1, tetA(A) were found in all studied strains, aadA1 in four strains and three strains exhibited the blaCTX-M gene. Therefore, according to the results, the recovered E. coli was endometrial pathogenic E. coli (EnPEC) with multiple antibiotic resistance. Furthermore, the virulence activities and phenotypic resistance to the antibiotics correlated strongly with the presence of the genes fimH, papC, blaTEM, blaCTX-M, aadA1, sul1, tetA(A) in these strains. | ||
| Keywords | ||
| Endometrial pathogenic Escherichia coli; endometritis; Congo Red; PCR | ||
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