Anticancer Activity of Curcumin Nanoparticles Against Lung Adenocarcinoma (A549) Cell Lines | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Articles in Press, Corrected Proof, Available Online from 08 October 2025 PDF (879.13 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2025.422243.1123 | ||
| Authors | ||
| Marwa M. Zaky1; K. A. Soliman1; A. A. A. Omran2; Neima K. Al-Senosy* 1 | ||
| 1Genetics Department, Faculty of Agriculture, Ain Shams University | ||
| 2Biological and Geological Sciences Department, Faculty of Education, Ain Shams University | ||
| Abstract | ||
| Curcumin nanoparticles (CNPs) was recognized for their therapeutic properties. Therefore, this research also aimed to investigate the anticancer properties of curcumin nanoparticles and the mechanism involved. The findings revealed that CNPs demonstrated antiproliferative activity and potent cytotoxicity against human lung adenocarcinoma cells (A549) with an IC50 value of 78.64 μg/mL. In contrast, these CNPs caused significantly negligible cytotoxic activities on normal lung cells (Wi38) at an IC50 value of 150.63 μg/mL. Furthermore, flow cytometric protocol using propidium iodide staining also evidenced that CNP treatment of A549 cells enhanced S-phase and G2/M phase cell cycle arrest. The results from the Annexin V/PI staining protocol evidenced increased apoptosis and necrotic cell death. The qRT-PCR protocol was employed to examine the mRNA expression of p53, Bcl-2, and caspase3 genes. The apoptosis pathway induced by CNPs involved the up-regulation of p53 and caspase3 and down-regulation of Bcl-2. The findings suggest that CNPs suppress cell growth by suppressing cell cycle at the S phase and inducing cytotoxicity in carcinoma cells through apoptosis mediation. Finally, these results indicate that nanocurcumin (CNPs) is a promising new strategy for cancer treatment, which can achieve greater efficacy with less toxicity than traditional therapies. | ||
| Keywords | ||
| Curcumin Nanoparticles; Lung Cancer; in vitro MTT assay; RT-PCR; Apoptosis and Cell line | ||
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