A Facile RP-HPLC Method for Quantification of Antiviral Drug Daclatasvir Dihydrochloride in Both Tablet Dosage Form and River Nile Samples | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Volume 29, Issue 5, September and October 2025, Pages 2079-2102 PDF (480.06 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2025.427072.6646 | ||
| Author | ||
| Mohammed et al. | ||
| Abstract | ||
| A rapid and sensitive RP-HPLC method was developed for the quantitative analysis of daclatasvir dihydrochloride in tablet dosage forms. The method utilizes a Zorbax Eclipse Plus C18 column and a mobile phase consisting of 0.02M KH2PO4 buffer (pH 3.0) and acetonitrile (70:30% v/v). The method demonstrated excellent linearity (R² > 0.9996) over the concentration range of 0.15- 90µg/ mL, with low limits of detection (LOD) and quantification (LOQ). The method was also accurate and precise, with recovery values ranging from 98.72 to 101.30% and relative standard deviations less than 2.0%. In conclusion, this validated RP-HPLC method provides a reliable and efficient tool for the routine analysis of daclatasvir dihydrochloride in tablet formulations and the Nile River samples. This method can be applied for various quality control purposes, including dissolution testing and biowaiver studies. | ||
| Keywords | ||
| Daclatasvir; HPLC; Vitro dissolution; stability-indicating; Assay; Biowaiver | ||
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