Assessment of the synergistic effects of essential oils and antibiotics on antibiotic-resistant food-borne disease bacteria in Burkina Faso. | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 09 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.351390.2466 | ||
| Authors | ||
| Paul SODERE* 1; Marius Kounbèsiounè SOMDA1, 2; Luc M.I ZONGO3, 4; Iliassou MOGMENGA2, 5; Henriette MIHIN1, 2; Agbémébia Yawovi AKAKPO1; Mamoudou Hama DICKO2 | ||
| 1Laboratory of Microbiology and Microbial Biotechnology, Department of Biochemistry and Microbiology, University Joseph KI-ZERBO, 03 BP: 7021, Ouagadougou 03, Burkina Faso | ||
| 2Laboratory of Biochemistry Biotechnology Food Technology and Nutrition, Department of Biochemistry Microbiology, University Joseph KI-ZERBO, 03 BP: 7021, Ouagadougou 03, Burkina Faso | ||
| 3Hospital Saint Camille of Ouagadougou (HOSCO), 09 BP 444 Ouagadougou 09, Ouagadougou, Burkina Faso | ||
| 4Faculty of Health Sciences, University Saint Thomas d’Aquin (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso | ||
| 5Banfora University Centre, University Nazi BONI, 01 BP 1091 Bobo-Dsso 01, Burkina Faso | ||
| Abstract | ||
| Background: Antibiotic resistance is a major public health concern in Burkina Faso, highlighting the urgent need for alternative therapeutic strategies. This study aimed to evaluate the in vitro synergistic effects of essential oils extracted from two medicinal plants in Burkina Faso, Hyptis suaveolens and Laggera aurita, in combination with ampicillin, amoxicillin, amoxicillin + clavulanic acid, and colistin) against antibiotic-resistant bacteria. Methods: The study assessed the antimicrobial activity of essential oils and antibiotics and that of their combination against seven bacterial strains: Staphylococcus aureus ATCC 2523, Enterococcus faecalis 0366 V, Escherichia coli ATCC 25922, Salmonella typhi SKN 1152, Salmonella paratyphi SKN 601, Yersinia enterocolitica 0938 V, Pseudomonas aeruginosa ATCC 9027. The agar diffusion well, microdilution, checkerboard, and kill curve methods were employed. Results: L. aurita essential oil showed a mean inhibition diameter of 37.83 ± 1.24 mm, with MIC values ranging from 0.59 to 7.97 mg/mL. H. suaveolens essential oil showed a mean inhibition diameter of 14.67 ± 0.40 mm, with MIC values ranging from 2.02 to 8.11 mg/mL. The combination of L. aurita and H. suaveolens essential oils with antibiotics exhibited synergistic effects in 64.7% and 60% of cases, respectively. The kill curve assay demonstrated the shortest bacterial killing time at 4 hours and the longest at 16 hours, depending on the synergistic combination. Conclusion: The study suggests that combining essential oils with antibiotics could serve as an alternative treatment for multidrug-resistant infections. Further investigations, including clinical trials, are necessary to confirm the therapeutic potential of these combinations. | ||
| Keywords | ||
| Antibiotics; bacterial resistance; essential oils; synergy; multidrug-resistant infections | ||
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