USING SODIUM AZIDE TO INDUCE MUTATIONS IN DIMORPHOTHECA ECKLONIS (L.) AND SRAP GENETIC MARKERS TO IDENTIFY THE GENETIC VARIANCE | ||
| Scientific Journal of Flowers and Ornamental Plants | ||
| Volume 12, Issue 3, September 2025, Pages 133-153 PDF (4.53 M) | ||
| Document Type: Original Research Article | ||
| DOI: 10.21608/sjfop.2025.385027.1036 | ||
| Authors | ||
| Makka Ali Hassan* 1; Yasser Ismail El-Nashar2; Mona AbdelHamid Sorour2 | ||
| 1Department of Horticulture (Ornamental plants), Faculty of Agriculture Desert and Environmental, Matrouh University, Egypt | ||
| 2Ornamental Plants and Landscape Gardening Research Department, Horticulture Research Institute, ARC, Alexandria, Egypt | ||
| Abstract | ||
| Dimorphotheca ecklonis (L.), commonly known as African daisy, is a half-hardy annual that produces large white blossoms. The flowers exhibit a striking contrast, being white with the disc florets presenting a deep blue or purple hue, a multifunctional plant that can be used in a variety of landscape designs. It is frequently utilized as a bedding plant, adding vibrant color to flower beds and borders. Additionally, it thrives in rock gardens and containers, allowing for flexible placement options. Its ability to withstand drought conditions makes it an excellent choice for low-water gardens, thus supporting sustainable landscaping practices. In the Nursery of Floricultural and Ornamental Plants, Faculty of Agriculture, Alexandria University, Egypt, the current investigation was conducted over the course of two consecutive seasons, 2022/2023 and 2023/2024. The seedlings (shoot tip) of Dimorphotheca ecklonis (L.) were sprayed with sodium azide at the levels of 1500, 3000, 4500, 6000 ppm and control (distilled water) to evaluate its effect on the vegetative, flowering growth and phytochemical composition, induction of mutation and genetic variability on Dimorphotheca ecklonis (L.) plants. Also, SRAP markers were used to evaluate the induced mutants. The levels of 6000 ppm considerably enhanced the plant height and dry weight of vegetative growth in M2-generation, number of branches and leaves in both generations. The concentration of 6000 ppm increased significantly the leaf area in both generations. The concentrations of 6000 and 3000 ppm in the M1and M2-generations, respectively, are the latest flowering. The level of 3000 ppm in both generations substantially elevated the levels of anthocyanin and beta-carotene. Some variations in the habit of growth, leaf form and inflorescence structure were observed in both generations. SRAP marker-PCR technique was able to detect mutations in Dimorphotheca ecklonis (L.) plants | ||
| Keywords | ||
| Dimorphotheca ecklonis; anthocyanin; phenols; beta carotene; ISSR marker | ||
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