Prevalence, antibiogram and enterotoxin production of Staphylococcus aureus in Nile Tilapia, Mullet and Catfish with a reduction trial using acetic and ascorbic acids.
Elsaady, H., Bakeir, N., Darwish, W., Ali, E. (2025). Prevalence, antibiogram and enterotoxin production of Staphylococcus aureus in Nile Tilapia, Mullet and Catfish with a reduction trial using acetic and ascorbic acids.. EKB Journal Management System, 13(1), 40-51. doi: 10.21608/djvs.2025.384892.1155
Hosny Abdlkader Elsaady; Nabil Bakeir; Wageh Sobhy Darwish; Eman Hamdy Ali. "Prevalence, antibiogram and enterotoxin production of Staphylococcus aureus in Nile Tilapia, Mullet and Catfish with a reduction trial using acetic and ascorbic acids.". EKB Journal Management System, 13, 1, 2025, 40-51. doi: 10.21608/djvs.2025.384892.1155
Elsaady, H., Bakeir, N., Darwish, W., Ali, E. (2025). 'Prevalence, antibiogram and enterotoxin production of Staphylococcus aureus in Nile Tilapia, Mullet and Catfish with a reduction trial using acetic and ascorbic acids.', EKB Journal Management System, 13(1), pp. 40-51. doi: 10.21608/djvs.2025.384892.1155
Elsaady, H., Bakeir, N., Darwish, W., Ali, E. Prevalence, antibiogram and enterotoxin production of Staphylococcus aureus in Nile Tilapia, Mullet and Catfish with a reduction trial using acetic and ascorbic acids.. EKB Journal Management System, 2025; 13(1): 40-51. doi: 10.21608/djvs.2025.384892.1155

Prevalence, antibiogram and enterotoxin production of Staphylococcus aureus in Nile Tilapia, Mullet and Catfish with a reduction trial using acetic and ascorbic acids.

Damanhour Journal of Veterinary Sciences
Volume 13, Issue 1, February 2025, Pages 40-51    PDF (914.29 K)
Document Type: Original Article
DOI: 10.21608/djvs.2025.384892.1155
Authors
Hosny Abdlkader Elsaady* 1; Nabil Bakeir2; Wageh Sobhy Darwish3; Eman Hamdy Ali4
1Demonstrator at Meat hygiene, Technology and food safety Department, faculty of vet med Damanhour University
2Professor of Infectious diseases,Faculty of veterinary medicine.Damanhour univerisity
3Professor of food hygiene, Fac. Vet. Med., Zagazig Univ., Egypt
4Food Hygiene and control department, Faculty of veterinary medicine, Damanhour university
Abstract
This study aimed to examine the prevalence of Staphylococcus aureus (S. aureus) in Nile tilapia, mullet, and catfish sold in El-Beheira governorate, Egypt. The antibiotic susceptibility of the isolated S. aureus strains was assessed. Further, the Reverse Passive Latex Agglutination technique (RPLA) was employed for detection and identification of S. aureus enterotoxins. The current results indicated the isolation of S. aureus from Nile tilapia, mullet, and catfish, with incidence rates of 60%, 45%, and 70%, respectively. Catfish exhibited a substantially higher overall S. aureus count (3.64±2.49 log10 cfu/g) as compared to Nile tilapia (3.44±2.27 log10 cfu/g) and mullet (2.00±1.85 log10 cfu/g), respectively (p< 0.05). Accurately, 55%, 35%, and 20% of the analyzed samples of catfish, Nile tilapia, and mullet, respectively, surpassed the Egyptian threshold for S. aureus (3.00 log10 cfu/g). The isolated S. aureus strains exhibited distinct multidrug resistance patterns. However, RPLA testing of specific S. aureus isolates for the presence of Staphylococcal enterotoxin indicated that enterotoxin A was identified in two S. aureus isolates obtained from catfish and in one isolate obtained from tilapia. Enterotoxin C was found solely in one strain obtained from mullet. Enterotoxin D was identified in a single sample from mullet and one strain from tilapia. Enterotoxin A+ D was found solely in one isolate obtained from catfish. Experiments aimed at decreasing S. aureus counts with 1% and 2% acetic acid and 1% and 2% ascorbic acid yielded reduction percentages of 21.2%, 46.1%, 17.3%, and 28.9%, respectively.
Keywords
S. aureus; Catfish; Tilapia; Mullet; antimicrobial resistance
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