Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies
Ben hammouda, M., El Ghali, F., Mnif, S., Siala, M., Attia, H., Azabou, S. (2025). Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies. EKB Journal Management System, 2(2), 1-21. doi: 10.21608/sasj.2025.390084.1007
Mouna Ben hammouda; Fares El Ghali; Sami Mnif; Mariam Siala; Hamadi Attia; Samia Azabou. "Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies". EKB Journal Management System, 2, 2, 2025, 1-21. doi: 10.21608/sasj.2025.390084.1007
Ben hammouda, M., El Ghali, F., Mnif, S., Siala, M., Attia, H., Azabou, S. (2025). 'Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies', EKB Journal Management System, 2(2), pp. 1-21. doi: 10.21608/sasj.2025.390084.1007
Ben hammouda, M., El Ghali, F., Mnif, S., Siala, M., Attia, H., Azabou, S. Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies. EKB Journal Management System, 2025; 2(2): 1-21. doi: 10.21608/sasj.2025.390084.1007

Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies

Spectrum Science Journal
Volume 2, Issue 2, November 2025, Pages 1-21    PDF (777.32 K)
Document Type: Original Article
DOI: 10.21608/sasj.2025.390084.1007
Authors
Mouna Ben hammouda* 1; Fares El Ghali2; Sami Mnif2; Mariam Siala3; Hamadi Attia1; Samia Azabou1
1Valuation, Analysis and Food Safety Laboratory (LAVASA), Engineering School of Sfax (ENIS), University of Sfax, 3038 Sfax, Tunisia
2Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia
3Laboratory of Environmental Sciences and Sustainable Development “LASED”, Sfax Preparatory Institute for Engineering Studies, University of Sfax, P.B 1171, Sfax 3000, Tunisia
Abstract
The surface disinfectant property of a homemade prickly pear vinegar (PPV) is investigated throughout this study, using in vitro testes assisted by a molecular docking study, against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Salmonella enterica) strains. Results show that PPV exhibited growth-inhibitory effect for all tested bacterial strains at MIC values ranging between 1.56, and 3.12% (v/v). Obtained results showed that PPV was more effective to inhibit initial cell attachment (86.33, 54.15, and 72.19% at 2×MIC) compared to 24h-preformed biofilms (67.64, 42.69 and 39.94% at 2×MIC). Treatment of abiotic surface with PPV at 2×MIC resulted in a significant reduction (p < 0.05) of bacterial metabolic activity (72.10, 52.92 and 56.96%, respectively). The anti-biofilm activity of PPV was also evaluated on two additional abiotic surfaces such as glass and stainless steel and results showed that treatment of biofilms with PPV at 2×MIC (% v/v) resulted in a log reduction of the initial biomass. Molecular docking analysis predicted the ability of phenolic compounds present in PPV, particularly, rutin to interact with SarA protein involved in S. aureus biofilm formation.
Keywords
Prickly pear vinegar; Antimicrobial activity; Anti-biofilm activity; Abiotic surfaces; Molecular docking
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