Ascorbic Acid Nanoparticles Play a Role in Promoting Salinity Resistance in Catharanthus roseus plants. | ||
| SVU-International Journal of Agricultural Sciences | ||
| Articles in Press, Accepted Manuscript, Available Online from 11 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/svuijas.2025.414746.1501 | ||
| Authors | ||
| Dina Salem Salem* 1; Hoda A.S. El-Garhy2; Diaa Abd El-Moneim3; Tahsin Shoala4 | ||
| 1Assistant professor at Department of Agriculture Biotechnology, College of Biotechnology, Misr University for Science and Technology | ||
| 2Genetics and Genetic Engineering Dept. Faculty of Agriculture, Benha University | ||
| 3Department of Plant Production, (Genetic Branch), Faculty of Environmental and Agricultural Sciences, Arish University, | ||
| 4Department of Environmental Biotechnology, College of Biotechnology, Misr University for Science and Technology | ||
| Abstract | ||
| Catharanthus roseus is a known therapeutic herb that possesses many terpenoid indole alkaloids. Salt stress is one of the environmental stressors which impact plants productivity, A range of solutions have been developed to combat the damaging impacts of salt stress. This study examined the impact of ascorbic acid (ASC) in both bulk and nanoform at concentrations of 0.05 and 0.1 mM on the salt stress resistance of Catharanthus roseus plants. At both levels, ascorbic acid (ASC) and ascorbic acid nanoparticles (ASC-NPs) suppressed the impacts of salinity on Catharanthus roseus plants. Plant height, biomass, and plant weights all rose significantly in response to 0.1 mM ASC-NPs. Furthermore, ASC-NPs (0.1 mM) treatment increased chlorophyll and carotenoid concentration in plant leaves. In response to ASC -NPs at 0.1mM, catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) activity increased to their greatest levels. The WRKY40 gene reached the highest expression level (58.89-fold increase) in C. roseus plants in response to salinity and 0.1 mM ASC-NPs treatment compared to the control. According to qRT-PCR data, WRKY1, WRKY2, and MYC2 genes were downregulated following treatment application, in comparison to the control, except for 0.1 mM ASC-NPs under salt stressor (150 mM NaCl), where the expression of LEA and MAPK6 genes increased (2.93-fold) above the control. | ||
| Keywords | ||
| Salinity; Catharanthus roseus; Ascorbic acid nanoparticles; qRT-PCR | ||
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