Molecular characterization of carbapenemase-producing Gram-negative bacilli isolated from clinical samples in Senegal | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 12 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.397723.2948 | ||
| Authors | ||
| Ibrahim Moustoifa* 1; Cheikh ibrahima LO2; Fatoumata Diallo3; Ousmane Sadio2; Moussa Moise Diagne4; Safiétou Sankhe4; Madiagne Der5; Yakhya Dieye2; Mouhamadou Lamine DIA3 | ||
| 1Département de Biologie Animale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, Dakar BP 5005, Sénégal | ||
| 2Pôle Microbiologie de l’Institut Pasteur de Dakar, Dakar, BP 220, Senegal | ||
| 3Faculté de Médecine, Pharmacie et Odonto-Stomatologie, Université Cheikh Anta Diop de Dakar, Dakar, BP 5005, Senegal | ||
| 4Pôle Virologie de l’Institut Pasteur de Dakar, Dakar, BP 220, Senegal | ||
| 5Laboratoire de Bactériologie-Virologie du Centre Hospitalier National Universitaire de Fann, Dakar, BP 5035, Senegal | ||
| Abstract | ||
| Background: Antimicrobial resistance (AMR) is a major threat to global public health. In Senegal, the emergence of carbapenem resistance among Gram-negative bacilli (GNB) is a growing concern. Methods: This retrospective study aimed to characterize carbapenemase genes in 50 GNB isolates resistant to ertapenem and/or imipenem, recovered from clinical samples at the University Hospital Center (CHNU) of Fann between October 2022 and March 2024. Bacterial identification was performed using VITEK 2 and MALDI-TOF MS, and antibiotic susceptibility testing was conducted by agar disk diffusion. Whole-genome sequencing (WGS), followed by bioinformatics analyses, enabled the detection of resistance genes. Results: Among the 50 isolates, E. coli (n=21; 42%) was the most frequent species, followed by K. pneumoniae (n=12; 24%), Proteus mirabilis (n=5; 10%), and Enterobacter hormaechei (n=5; 10%). Carbapenemase genes were identified in 35 isolates, with blaNDM-5 (n=12; 34.3%) and blaNDM-1 (n=5; 14.3%) being the most prevalent. Other detected genes included blaOXA-181 (n=4; 11.4%) and blaOXA-484 (n=4; 11.4%), as well as co-occurrences such as blaNDM-5 + blaOXA-181 (n=3; 8.6%). We also report, for the first time in Senegal, the identification of blaNDM-15, blaOXA-68, blaOXA-70, blaOXA-420, and blaOXA-484 variants. Conclusion: These findings highlight the alarming dissemination of carbapenemases including newly reported variants in Senegal among clinical GNB isolates. They underscore the urgent need to strengthen surveillance strategies targeting multidrug-resistant bacteria in Senegal. | ||
| Keywords | ||
| Carbapenemases; Gram-negative bacilli; antimicrobial resistance; whole-genome sequencing; Senegal | ||
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