Impact of Integrating Two Activation Techniques with Nano Chitosan Irrigation on Antibacterial Efficacy. (An In Vitro Study) | ||
| Egyptian Dental Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 13 October 2025 PDF (406.28 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2025.406816.3564 | ||
| Authors | ||
| Fatma Ahmed Shokry* 1; Shams Waz Amgad2; Dalia Ali Moukarab3; Mohamed A. Mawhoup4 | ||
| 1Endodontic Department, Minya University, Egypt. | ||
| 2Assistant Professor, Crown and bridge department, Faculty of Dentistry, Minia University | ||
| 3Associate Professor, Endodontics, Faculty of Dentistry, Minia University, Egypt | ||
| 4Microbiology and Immunology , University of Deraya , Minia , Egypt | ||
| Abstract | ||
| Abstract Introduction: The purpose of this study was to compare in vitro study the impact of activation of nano chitosan irrigation with two different techniques on antibacterial efficacy on root canals infected with two different specimens. Aim of study: to asse and contrast impact of integrating passive ultrasonic irrigation or laser activation with nanochitosan against double species biofilm. Methods: A total of 50 extracted single-rooted teeth were sectioned at the cementoenamel junction, instrumented, sterilized using autoclave, and randomly assigned into five equal groups (n = 10) according to the designated antimicrobial protocols. All groups were inoculated with Enterococcus faecalis and staphylococcus aureus except Group I: (Negative control group) Autoclaved specimens in this group received no additional procedures, ensuring that any microbial growth could be attributed to contamination rather than procedural variables. Group II: No additional procedures were performed in the positive control group. Group III: specimens were irrigated with 0.2% chitosan nanoparticles (CNPs) with conventional needle irrigation (CNI). Group IV: specimens received irrigation with 0.2% CNPs and passive ultrasonic irrigation (PUI). Group V: specimens were irrigated with 0.2% chitosan nanoparticles and laser activation (LAI). Microbiological testing (colony forming unit counting) was performed to evaluate and show, respectively, the effectiveness of the treatments. Data were subjected to One Way ANOVA test for comparison of quantitative data between the five groups followed by post hoc LSD analysis. Conclusions: Both PUI and LAI groups by using 0.2% nano-chitosan demonstrated success in reducing root canal infection compared with conventional irrigation group. | ||
| Keywords | ||
| Laser activation; Passive ultrasonic activation; Enterococcus faecalis | ||
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