EFFECT OF EXTRACTION METHODS ON PROTEIN ISOLATE YIELD AND FUNCTIONAL PROPERTIES OF SOME LEGUMES | ||
| Egyptian Journal of Agricultural Research | ||
| Volume 71, Issue 2, June 1993, Pages 529-538 PDF (2.53 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.1993.459048 | ||
| Authors | ||
| AHMED M. KHORSHID; MERVAT A. OSMAN; SANIA A. HASSAN | ||
| Food Technology Research Institute, Agricultural Research Centre, Giza, Egypt. | ||
| Abstract | ||
| Three local samples of unsound (damaged soy - bean), decorticated lentil and broad bean (Vicia faba) were used as source of protein in this study. Ca (OH)2 and NaOH methods were applied in protein isolate extraction. Protein extractability and precipitabifity were determined and results showed that NaOH method gave higher extractability than ca (OH)2 method whil the precipitation value showed a reverse trend. Results also show that broad bean residue after extraction of protein contain a pronounced amount of carbohydrate as strach , while soy - bean and lentil still had a relatively good balance of protein and carbohydrate contents and lentil residues could be used as good and well balanced feed stuff while broad bean might be used as a source of starch production. It could be concluded also that both extraction methods produce protein isolates with a very close purity which determined by the nitrogen content or crude protein of the isolates. Generally , results show that the method of protein extraction had a little effect on the protein functional properties except that of the coagulated protein percent which increased in case of using NaOH, method . As a conclusion the three damaged and unsound samples could provide a good source of absorption capacity , which suggests potential use of these proteins as a meat binder or extender, and also in doughs and baker products. | ||
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