EFFECT OF ADDING L-ARGININE TO TRIS-YOLK EXTENDER OF SUB-FERTILE EJACULATES IN RAMS | ||
| Journal of the Egyptian Veterinary Medical Association | ||
| Volume 84, Issue 1, April 2024, Pages 7-17 PDF (525.05 K) | ||
| Document Type: Original Research Articles | ||
| Abstract | ||
| Forty ejaculates of poor motility (50 to 55%) were collected from 8 rams and extended with Tris-yolk extender (1:4) supplemented with varying amounts of L-arginine (0, 2, 4 and 6 mM) in order to study the effect of L-arginine supplementation on sub-fertile ram ejaculates. Computer-assisted semen analysis was used to assess semen samples that were incubated at 5oC for 4 hours. The findings indicated that, in comparison to the control semen extender, the addition of 6 mM L-arginine increased (P<0.05) the percentages of total and rapidly progressing sperm motility while decreasing the percentages of non-motile and static sperm. In comparison to L-arginine free extender, the addition of L-arginine (4 and 6 mM) increased the percentages of vitality and normalcy and decreased the percentages of aberrant neck and tail. No significant (P>0.05) change was seen in the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in semen extender upon addition of all L-arginine doses. After four hours of cold-temperature incubation, the ejaculate of a sub-fertile ram can have improved sperm motility, velocity, and vitality by supplementing the Tris-yolk extender with L-arginine (4 or 6 mM) | ||
| Keywords | ||
| Arginine. Rams. CASA. Poor Semen; Subfertility | ||
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