Preparation of water-soluble O-(succinyl) chitosan derivatives and their antimicrobial activity against some plant pathogens | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 18, Issue 1, 2010, Pages 51-68 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.2010.459107 | ||
| Author | ||
| Entsar I. Rabea* | ||
| Department of Pest Control and Environmental Protection; Faculty of Agriculture, Damanhour, Alexandria University, Egypt | ||
| Abstract | ||
| In the present study, several water soluble O-(succinyl) chitosan derivatives were synthesized to study their enhancement of the antimicrobial activity of chitosan molecule. The chemical structures of the compounds were characterized by 1H-NMR spectroscopy and their degree of substitution ranged from 0.18 to 0.64. The antimicrobial activity was investigated against bacteria of crown gall Agrobacterium tumefaciens and soft mold Erwinia carotovora and fungi of early blight Alternaria alternata, grey mold Botrytis cinerea and root rot Fusarium oxysporum. O-(succinyl) chitosans showed much more enhancement the antibacterial activity than chitosan. A derivative with a DS of 0.18 was the most active with MIC = 600 and 525 mg.L-1 against A. tumefaciens and E. carotovora, respectively. The effects of these derivatives were also assessed on polygalacturonase and pectin-lyase activities in the tested bacteria. O-(succinyl) chitosan with a DS of 0.64 caused a high inhibition of the above enzymes activities at 250, 500 and 1000 mg.L-1. The study showed that O-(succinyl) chitosans were much more potent as antifungal activity than chitosan and a compound of 0.64 DS was the highest in its mycelial growth inhibition with EC50 of 1380, 1945 and 1505 mg.L-1 against A. alternata, B. cinerea and F. oxysporum, respectively. The study showed that the spore germination of B. cinerea and F. oxysporum was significantly affected with the above chitosan derivatives at 50, 125 and 250 mg.L-1 and the inhibition activity was increased with an increase in the DS. | ||
| Keywords | ||
| O-(succinyl) chitosan; 1H-NMR spectroscopy; antibacterial activity; antifungal activity; fungal spore germination; polygalacturonase; pectin-lyase | ||
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