Assessment of The Expression of Both knj12 and znf132 Genes as Potential Non-Invasive Plasma Markers for Early Detection of Different Colonic Neoplasia | ||
| Benha Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 16 October 2025 PDF (642.78 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bmfj.2025.404531.2546 | ||
| Authors | ||
| Mahmoud M Morgan* 1; Badawy A Abdulaziz2; Awad S.A A.2; Mahmoud M sheded3; Naglaa F Alhusseini4; Khaled Nasef5; Hesham M Elwakeel6; Mona S Abdelhamid7 | ||
| 1Department of Hepatology, Gastroenterology and Infectious diseases, Msc of Tropical Medicine – Faculty of Medicine - Suez University | ||
| 2Department of Hepatology, Gastroenterology and Infectious Diseases - Faculty of Medicine - Benha University | ||
| 3Department of Infectious Diseases, Hepatology and Gastroenterology, Faculty of Medicine - Suez University | ||
| 4Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine - Benha University | ||
| 5Department of Oncology Unit, Internal Medicine Department, Faculty of Medicine – Mansoura University | ||
| 6Department of Hepatology,Gastroenterology Department- Ahmed Maher teaching hospital- GOTHI | ||
| 7Department of Hepatology, Gastroenterology and Infectious Diseases, Faculty of Medicine- Benha University | ||
| Abstract | ||
| Background: Colorectal cancer (CRC) is one of the most prevalent cancers globally and a main cause of cancer-related death. Current diagnostic methods like colonoscopy, while effective, suffer from low patient compliance due to invasiveness. Hence, there is a critical need for sensitive and non-invasive biomarkers for early detection. Aim: This research aimed to assess the plasma expression levels of the knj12 and znf132 genes as potential non-invasive markers for early detection of different forms of colonic neoplasia. Methods: This prospective case-control research has been carried out on 80 participants. Group I included 55 patients with colonic polypoidal lesions (CRC, advanced adenoma, and serrated sessile adenoma), while Group II included 25 healthy controls. Gene expression was analyzed and correlated with lesion characteristics and histopathology. Results: There was a statistically significant positive association between knj12 and znf132 expression across all neoplastic subgroups. At cutoff values of 1.13 (knj12) and 1.05 (znf132), both genes demonstrated moderate sensitivity and specificity for CRC detection. ZNF132 showed higher diagnostic performance in serrated sessile adenomas (sensitivity 87%, specificity 60%). Combined testing improved early neoplasia detection and correlated with lesion histopathology and classification. Conclusion: KNJ12 and ZNF132 show promising potential as non-invasive plasma biomarkers for early detection of colonic neoplasia. Their combined evaluation could serve as a valuable adjunct to current screening strategies, especially for patients with low compliance to invasive procedures like colonoscopy. | ||
| Keywords | ||
| Colonic Neoplasia; knj12; znf132; gene expression; non-invasive biomarkers | ||
|
Statistics Article View: 3 PDF Download: 2 |
||
