Efficacy of sequential spray of some essential oils against faba bean chocolate spot disease under laboratory and field conditions | ||
Journal of Pest Control and Environmental Sciences | ||
Volume 15, Issue 1, June 2007, Pages 1-19 PDF (257.89 K) | ||
Document Type: Original Article | ||
DOI: 10.21608/jpces.2007.459642 | ||
Authors | ||
M. M. H. Rahhal* 1; A. A. Hussien2 | ||
1Plant Pathology Institute, Agric. Res. Center, Giza, Egypt | ||
2Central Agric. Pes. Lab., Agric. Res. Center, Dokki, Egypt | ||
Abstract | ||
Studies were carried out in vitro and in vivo to determine the antifungal activity of different sequential spray systems of two essential oils; clove and rose in addition to the fungicide Mancozeb against faba bean chocolate spot disease which caused by Botrytis fabae. In vitro, results revealed that both of essential oils at five tested concentrations; 25, 50, 100, 250 and 500 ppm decreased the mycelial growth of B. fabae. Clove and rose oils and Mancozeb at 500 ppm completely inhibited the fungal growth up to 7 days from inoculation. Also, conidia germination % and its germ tube length (µm) decreased by increasing oil concentrations and the best reduction effect was cleared in the case of 500 ppm for both tested oils. Clove oil reduced conidia germination and germ tube length from 0.52 to 79.59 % and from 6.29 to 56.19 %, respectively and at the same time rose oil reduced it from 3.59 to 95.39% and 29.22 to 66.33%, respectively according to the oil concentrations used. In vivo, faba bean chocolate spot disease severity was decreased in faba bean cultivars; Giza 843, Misr 1, Sakha 1 and Nubaria 1 by using sequential spray system Mancozeb / Mancozeb followed by Rose / Mancozeb and Rose / Rose in both growing seasons. Pods number / plant and seeds number / plant were the highest in case of Mancozeb / Mancozeb followed by Rose / Mancozeb but this sequence was upside down with seeds weight / plant. Also, the other sequential spray systems containing rose oil had promising results. | ||
Keywords | ||
Faba bean; cultivars; chocolate spot; essential oils; clove oil; rose oil | ||
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