Isolation and Identification of Major Pathogenic Bacteria from different hospital patients | ||
| Mansoura Journal of Biology | ||
| Volume 60, Issue 7, December 2022, Pages 1-5 PDF (1.04 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2022.459786 | ||
| Authors | ||
| Elzahraa M. Elazab1; Hoda M. Soliman1; Moustafa M. El-Shaer2; Yasser El-Amier* 3 | ||
| 1Botany Department, Faculty of Science, Mansoura University, Mansoura - 35516, Egypt | ||
| 2Fellow of Microbiology Medical Specialized Hospital, Mansoura University | ||
| 3Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt | ||
| Abstract | ||
| The present study aimed to isolation and identification of major pathogenic bacteria from different hospital patients. The bacterial isolates were characterized morphologically according to colony shape, margin, elevation, texture, pigmentation. A total of 120 clinical samples were collected during the 2021 year from Mansoura University Hospital. The clinical samples included blood (40), urine (35), and stool (45) from different patients with different genders and ages ranging from (16-55 years). In the present result, 95 samples had bacterial growth after incubation, on the other side 25 samples did not indicate any growth. all the colony’s shape was circular except strain 5 was irregular in shape. All colonies had an entire margin, raised elevation, creamy texture; and different color (off-white, pale yellow, yellowish – white, and orange) of pigmentation. The examined microscopically for cell shape, which revealed that the majority of isolates were rod shaped except strain 3 were cocci shape; Gram strain whereas the samples showed that majority strains are Gram negative but strain 3and 6 are Gram – positive and motility whereas the samples showed all strains that could motile except strains (2,3 and 8) are non-motile | ||
| Keywords | ||
| Pathogenic Bacteria; Isolation, Identification, Deseases, E. coil, | ||
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