Effect of Sodium Bicarbonate on the Cellular Morphology of Mycobacterium tuberculosis by Using the Scanning Electron Microscope | ||
| Mansoura Journal of Biology | ||
| Volume 60, Issue 7, December 2022, Pages 61-68 PDF (1.15 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2022.459793 | ||
| Authors | ||
| Aya G. Elgohary* 1; Mohammad K. ElBadrawy2; Heba E. Eldegla3; Gamal M. Abdel-Fattah1 | ||
| 1Microbiology and Botany Department, Faculty of science, Mansoura University, Mansoura, Egypt. | ||
| 2Chest Department, Faculty of Medicine, Mansoura university, Mansoura, Egypt. | ||
| 3Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura university, Mansoura, Egypt. | ||
| Abstract | ||
| There is a fundamental need to consider alternative anti-TB drugs because the emergence of multi-drug resistant (MDR) and extensively-drug hardy (XDR) strains of Mycobacterium tuberculosis has made the problem of tuberculosis control more challenging. This study was aimed to detect the effect of sodium bicarbonate 8.4% and some medicinal plants on the cellular morphology of M. tuberculosis by using the scanning electron microscope. Method: This study was performed on 30 M. tuberculosis isolates from different samples, 20 isolates (17 sputum and 3 bronchoalveolar lavage) from patients who were admitted to the chest department in Mansoura University Hospitals and 10 M. tuberculosis cultures from Central Public Health Laboratories in Cairo. Isolation and identification of M. tuberculosis. Determination of minimum inhibitory concentration (MIC) of ethanolic plant extracts by using resazurin microtitre plate assay (REMA). Then the effect of sodium bicarbonate 8.4% and garlic at MIC=3.25 mg\ml on the cellular morphology of M. tuberculosis by using the scanning electron microscope. Results: Sodium bicarbonate of 8.4% has a destructive effect on INH Isoniazid-resistant M.tuberculosis as indicated in the scanning electron micrographs. Garlic at MIC=3.25 mg\ml has an inhibitory effect on INH Isoniazid-resistant M. tuberculosis. Conclusions: Sodium bicarbonate and garlic can be used as adjuvant drug to treat INH Isoniazid-resistant M. tuberculosis with the antituberculosis drug. | ||
| Keywords | ||
| Mycobacterium tuberculosis; tuberculosis (TB); sodium bicarbonate (SB); resazurin microtiter plate assay (REMA); Allium sativum | ||
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