Isolation, identification and antibiotic susceptibility of Vibrio parahaemolyticus isolated from shrimp aquaculture | ||
| Mansoura Journal of Biology | ||
| Volume 61, Issue 8, December 2022, Pages 32-41 PDF (1.24 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2022.459807 | ||
| Authors | ||
| Abdallah. M* 1; Aida, M. Farag2; Abdel-Fattah G.M3 | ||
| 1Director of the Ghaliuon Research and Development Center , Egypt. | ||
| 2Marine Biotechnology and Natural Products Extract Lab., National Institute of Oceanography and Fisheries, Alexandria Branch, Egypt | ||
| 3Department of Botany, Faculty of Science, Mansoura University, Egypt. | ||
| Abstract | ||
| Vibrio parahaemolyticus is a chief foodborne pathogen accountable for main monetary damages in aquaculture and a peril to social wellbeing. V. parahaemolyticus was isolated from private aquaculture farms in the delta region in Kafr El-Sheikh Governorate, Egypt. A total of 160 examined Mugil capito (Chelon ramada), sea bass (Dicentrarchus labrax), sea bream (Sparus aurata) and Penaeus vannamei (shrimp) samples were collected from the aquaculture arrangement. 25% of samples were positive for V. parahaemolyticus. Shrimp agronomy faced grave glitches with diseases caused by V. parahaemolyticus, which have been frequently associated with fatalities both, in hatcheries and growout pools. The Vibrio species isolates from infected shrimp samples were biochemically and molecularly identified. Results revealed that, only 5 (12.5%) isolates from Penaeus vannamei (shrimp) were found positive for the thermostable direct hemolysin (tdh) and the the rmost able direct hemolysin-related hemolysin virulence factor genes (trh). The isolates were found to be 99% identical to the position strain V. parahaemolyticus and were deposited on GenBank under the accession number (OM654368). The antibiotic sensitivity test of the virulent strain of V. parahaemolyticus MAA3 showed highly resistance to ampicillin (10 µg) , amikacin (30 µg), cefotoxime (30 µg), ceftazidime (30 µg), and intermediate sensitivity to Erythromycin (15 µg). On the other hand, it was highly sensitive to ceftriaxone (30 µg), chloramphenicol (30 µg), cotrimoxazole (25 µg), trimethoprim (1.25 µg), sulfamethoxazole (23.75 µg), ciprofloxacin (5 µg), doxycycline HCl (30 µg), gentamycin (10 µg), levofloxacin (5 µg), tetracycline (30 µg), and tobramycin (10 µg). This study showed that Vibro parahaemolyticus have the ability to resist several antibiotics in addition to being very sensitive to other antibiotics, and the presence of virulence factors in Vibro parahaemolyticus represents a major threat to human health and economic resources in the future. To improve seafood safety, it is necessary to continuously evaluate the antibiotic and molecular features of V. parahaemolyticus | ||
| Keywords | ||
| Vibrio parahaemolyticus; aquaculture; Shrimp; isolation; antibiotic | ||
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