Stability Study of Four Isolated E. coli O157 Phages and Influence of Various Carriers on Their Cocktail effectivity | ||
| Egyptian Journal of Medical Microbiology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 April 2026 | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.427032.1893 | ||
| Authors | ||
| Aya M. Rabie1; Reda M. Taha1; Khalid El-Dougdoug2; Amany M. Reyad1 | ||
| 1Department of Botany, Faculty of Science, Fayoum University | ||
| 2Faculty of Agriculture, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| Background: Escherichia coli O157 is a pathogen that induces severe gastrointestinal illnesses with potentially life-threatening consequences, posing a threat to public health. Phage therapy, which employs bacteriophages to target and destroy bacteria specifically, is a potential alternative or complement to traditional antibiotic treatment. Objectives: This study employed the biocontrol of the most resistant E. coli O157 using a phage cocktail of four lytic phages. Methodology: This study involved detection, isolation, and characterization of four bacteriophages targeting an E. coli O157 isolate and the effect of three different carriers on their stability. Results: Plaque morphology and TEM micrographs revealed that two phages are from Myoviridae, one is from Siphoviridae, and the other is from Podoviridae. Conclusion: Our findings indicated that the phage cocktail demonstrated higher stability till 80ºC and 15% NaCl, and its viability was recorded in both acidic and alkaline conditions. Phage cocktail was mixed with three carriers, royal jelly, sorbitol, and trehalose in 1:1 (V: W%). By studying the stability of all formulas over time, trehalose was introduced as the best carrier, making it the preferred choice for application. | ||
| Keywords | ||
| E. coli O157; phage cocktail; carriers | ||
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