Fungal production of uricase on poultry wastes | ||
| Mansoura Journal of Biology | ||
| Volume 52, Issue 3, October 2021, Pages 10-15 PDF (917.21 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjb.2021.460015 | ||
| Authors | ||
| Nora A. Ebrahim* ; G. M Abdel-Fattah; A.H. Mowafy; A.S Gebreil | ||
| Department of Botany, Faculty of Science, Mansoura University, Egypt | ||
| Abstract | ||
| The main objective of this study was the isolation of fungi inhabiting different sites of poultry wastes and find out the most active fungi in uricase production. Among the tested strains of fungi using the plate assay method, Trichoderma sp, Mucor spinosus, Aspergillus terrus and Aspergillus flavus were the most active fungal strains, which were able to produce uricase. These fungal strains were tested for uricase production under submerged fermentation conditions. The highest uricase activity in liquid medium containing uric acid was obtained from Aspergillus flavus (7.5 U/mL), Aspergills terreus (4.6 U/mL) and Trichoderma sp. (2.6 U/mL) . It was clear that Aspergillus flavus was the most active fungus and was selected for further studies. The optimum conditions for maximum A. flavus uricase production were at a temperature of 30°C, pH 6.5 ,sucrose concentration of 10 g /L and NH4NO3 concentration of 17.5 g/l. Purification of the enzyme was carried out to give the enzyme with greatest yield by comparing the enzyme activity with the total activity in the original extract | ||
| Keywords | ||
| Fermentation; poultry wastes; Enzyme production; Optimization of uricase production; Aspergillus flavus | ||
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