Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum
Samir, N., Khalifa, M., El-Morsi, A., Abdel-Fattah,, G. (2021). Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum. EKB Journal Management System, 51(2), 1-7. doi: 10.21608/mjb.2021.460044
Nesma M. Samir; Mahmoud E. Khalifa; A. A. El-Morsi; G.M Abdel-Fattah,. "Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum". EKB Journal Management System, 51, 2, 2021, 1-7. doi: 10.21608/mjb.2021.460044
Samir, N., Khalifa, M., El-Morsi, A., Abdel-Fattah,, G. (2021). 'Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum', EKB Journal Management System, 51(2), pp. 1-7. doi: 10.21608/mjb.2021.460044
Samir, N., Khalifa, M., El-Morsi, A., Abdel-Fattah,, G. Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum. EKB Journal Management System, 2021; 51(2): 1-7. doi: 10.21608/mjb.2021.460044

Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum

Mansoura Journal of Biology
Volume 51, Issue 2, June 2021, Pages 1-7    PDF (634.96 K)
Document Type: Original Article
DOI: 10.21608/mjb.2021.460044
Authors
Nesma M. Samir* 1; Mahmoud E. Khalifa2; A. A. El-Morsi1; G.M Abdel-Fattah,1
1Department of Botany, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt
2Botany and Microbiology Department, Faculty of Science, Damietta University, Damietta, Egypt
Abstract
Trichoderma spp. are used extensively in agriculture as biological control agents to prevent soil-borne plant diseases. Seventeen fungal species were isolated from different sites of four governorates Dakahlia, Al-Sharqiya, kafr-Elsheikh and Damietta. The count of each fungal isolate was generally affected by physicochemical features of collected soils during the studies. The isolated fungal strains were morphologically classified into three classes namely; Zygomycetes, Ascomycetes and Hyphomycetes. Hyphomycetes was the most predominant class and was represented by three genera namely, Alternaria, Fusarium and Trichoderma. Ascomycetes was the second dominant class and was represented by two genera namely Aspergillus and Penicillium. Zygomycetes was represented by only one genera namely Rhizopus. Among all fungal isolates of this study, T. hamatum contained a single double-stranded RNA (dsRNA) segment of approximately 3 kb, which is typical of mycoviruses. We designated this mycovirus as Trichoderma hamatum mycovirus 1(ThaMV1). Transmission electron microscopy of (ThaMV1) revealed the presence of putative virus-like particles (VLPs) with an isometric structure approximately of 50 nm in diameter. This study represents the first report of isolation of a new unclassified mycovirus infecting T. hamatum
Keywords
Fungi; Physicochemical properties; Double-stranded RNA; Mycovirus
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