Assessment of Biotin (vitamin H) supplementation to sperm preparation medium on outcomes of intracytoplasmic sperm injection (ICSI). | ||
| Labyrinth: Fayoum Journal of Science and Interdisciplinary Studies | ||
| Articles in Press, Accepted Manuscript, Available Online from 19 October 2025 PDF (1.22 M) | ||
| Document Type: Original full papers (regular papers) | ||
| DOI: 10.21608/ifjsis.2025.425947.1132 | ||
| Authors | ||
| Wessam Salim Tawfik1; Samah Mamdouh Mamdouh Mohamed* 1; Eman Anwar Hassan2; Fatma Hany Abdulhady3; Mohamed Abd Elrahman Mahmoud Abd Elrahman4; Ahmad Ali Kandeel5 | ||
| 1Zoology Department, Faculty of Science, Fayoum University, Egypt. | ||
| 2Assistant professor of embryology. International Islamic Centre for Population Studies and Research, Al-Azhar University | ||
| 3Senior Embryologist wafa center El fayoum | ||
| 4Cell Biology Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt | ||
| 5Assistant Professor of Embryology-Comparative Anatomy, Faculty of Science, Zoology Department, Fayoum University | ||
| Abstract | ||
| Improving sperm motility is essential for infertility treatment and the efficacy of assisted reproductive technology (ART). This study examines the effects of biotin in sperm wash medium on motility and early embryonic development. Semen samples from 150 human male patients aged 21-45, with low motility (Asthenozoospermia and Necrozoospermia), were divided into three groups: pentoxifylline (PTX), SpermMobil and biotin (study group). The optimal biotin ratio was determined through comet assay analysis and evaluated using computer-aided sperm analysis (CASA) before and after treatment. All groups underwent intracytoplasmic sperm injection (ICSI) to compare fertilization, cleavage, and pregnancy rates. A biotin ratio of 30:100 micromolar (µM) was found to enhance sperm DNA integrity and significantly improve motility and velocity. While biotin, PTX, and SpermMobil all enhanced motility, the biotin group exhibited better fertilization and cleavage rates, as well as higher pregnancy rates. Our findings suggest that incorporating biotin in sperm wash medium at a 30:100 ratio notably improves reproductive outcomes compared to PTX and SpermMobil. | ||
| Keywords | ||
| Biotin; Sperm Motility; ICSI; Pentoxifylline; SpermMobil | ||
|
Statistics Article View: 1 |
||
