Unveiling the Impact of Hydroxyproline Supplementation Diet on Haemato-biochemical Aspects of Gilthead Seabream (Sparus aurata) Exposed to Ammonia Challenge Under Different Stocking Densities | ||
| Egyptian Journal of Aquatic Biology and Fisheries | ||
| Volume 29, Issue 5, September and October 2025, Pages 2779-2807 PDF (560.78 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejabf.2025.425346.6611 | ||
| Authors | ||
| Abdel-Salam A. Abou Ellif1; Abd El Rahman A. Khattaby2; Mohamed Sh. Abu Husein3; Hassan M.M. Khalf-Allah4; Mahmoud Mahrous M. Abbas* 3 | ||
| 1Zoology and Entomology Dept., Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt. | ||
| 2Fish Production and Aquaculture systems Department, Central laboratory for Aquaculture, Agriculture Research Center, Egypt | ||
| 3Zoology and Entomology Depart., Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt. | ||
| 4Zool. Dept., Fac. Sci., Al-Azhar Univ., Cairo, Egypt | ||
| Abstract | ||
| This study investigated the effects of dietary hydroxyproline supplementation on the haematological and biochemical responses of Sparus aurata fingerlings reared under two stocking densities (low: 15 fish/m3; high: 30 fish/m3) for 90 days, followed by an acute ammonia challenge. Four diets were prepared with increasing hydroxyproline levels (0, 1, 2, and 3%). Prior to ammonia exposure, significant improvements in red blood cell count, haemoglobin concentration, and haematocrit were observed in supplemented groups, with the highest values recorded at the 3% inclusion level. Concurrent reductions in mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH), along with elevated mean corpuscular haemoglobin concentration (MCHC), indicated adaptive adjustments in erythrocyte morphology. Platelet counts showed variable patterns, whereas white blood cell counts decreased with higher supplementation, suggesting an immunomodulatory role. Biochemical analyses revealed that hydroxyproline supplementation increased serum total protein, albumin, and globulin, while reducing glucose and cortisol concentrations, particularly under high-density conditions, reflecting improved metabolic stability and reduced stress. After ammonia exposure, most haematological and biochemical parameters declined across all groups, though reductions were less severe in the hydroxyproline-supplemented treatments, confirming its protective effect. In conclusion, dietary hydroxyproline at 3% inclusion provided the most consistent improvements in blood health, biochemical stability, and resilience to ammonia stress in S. aurata fingerlings. Therefore, a 3% hydroxyproline supplementation diet is recommended for practical application in intensive aquaculture systems. | ||
| Keywords | ||
| Seabream, Ammonia stress, Rearing density; Haematological parameters, Biochemical indices, Acute ammonia, Aquaculture | ||
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