FLAVONOID CONTENTS OF NEOTORULARIA TORULOSA AND ITS ANTIVIRAL ACTIVITY AGAINST COXSACKIEVIRUS B4 IN VITRO. | ||
| Advances in Basic and Applied Sciences | ||
| Volume 6, Issue 1, October 2025, Pages 41-47 PDF (1.56 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/abas.2025.410554.1073 | ||
| Author | ||
| Nawal H Hassan* | ||
| Medicinal and aromatic plants Depart. Desert Research Centre | ||
| Abstract | ||
| Viral infections are the most catastrophic problem that affects living standards. This research aims to discover and evaluate new antiviral agents from plant sources. Antiviral activities of total alcoholic extract of Neotorularia torulosa were examined in vitro against Coxsackievirus B4 (CoxB4) using MTT assay. The MNTC (maximum nontoxic concentration) of plant extract was 62.5 µg/ ml. The CC50% (50% cytotoxic concentration) of Neotorularia torulosa plant extract different concentrations (1000, 500, 250, 125, 62.5, 31.25, 15.62 and 7.81 ug/mL) on Vero cells was found to be 192.01 ± 1.01 µg/mL while the IC50 (50% inhibitory concentration) against Coxsackievirus B4 (CoxB4) was 23.069% ± 0. 067 µg/mL. The antiviral activity of the plant extract reached 95.15 % at the maximum nontoxic concentration compared with 77.71 for Acyclovir drug indicating that the plant extract exhibit promising antiviral activity against CoxB4. Seven flavonoid compounds were isolated from Neotorularia torulosa plant extract using various chromatographic techniques, including column chromatography, paper chromatography, and thin-layer chromatography (TLC). Final purification of flavonoid compounds was done using high-performance liquid chromatography (HPLC). Identification was carried out using different spectroscopic Analyses including UV and nuclear magnetic resonance (NMR) spectroscopy (¹H and ¹³C-NMR). | ||
| Keywords | ||
| flavonoids; Neotorularia torulosa, Antiviral; Coxsackievirus B4 (CoxB4) | ||
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