Effect of some pesticide on chitinase enzyme extracted from different insects | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 8, Issue 3, December 2000, Pages 59-70 PDF (674.41 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.2000.460576 | ||
| Authors | ||
| Nader shaker* ; M. Morshedy; S Mansour | ||
| Dept. of Pesticide chemistry, College of Agriculture, University of Alexandria, Alexandria, Egypt | ||
| Abstract | ||
| Chitinase (poly -1m4-(2-acetamido -2- deoxy) – D- ghcoside Glycanohydrolases EC 3.2.1.14) was extracted from Culex pipenes, Spodoptera littoralis larval stage and Musca domestica, Periplancta americana adult stage. The optimum condition for maximum chitinase activity were, at 4.0 pH,35ºC as a reaction temperature using chitin (an insduble linear B-1,4-linked polymer of N-acetylghucosamine) as enzyme substrate. An attempt was made to evaluate the ability of the tested Pesticides to inhibit the chitinase activity from different sources. The I50 values of diuron were 4×10-5, 3×10-5, 3.7×10-5 and 6×10-5M for S. littoral, P. americana, M. domestica and C. pipienes respectively, and the I50 values of chloropropham were 3.7×10-5, 4.6×10-5, 5.1×10-5 and 1×10-4M for S. littoralis, P. americana, M. domestica and C. pipienes, respectively. However, propanil incase the chitinase activity more than control. On the other hand, the percent of chitinase inhibition by deltametrin, methocarb, aldicarb, paraoxon and DNOC were 21, 20, 18, 17 and 15 respectively. The data shows interactive of different compounds studied on some biological parameters of treated insects depending mainly on the effect of these compounds on the chitin biosynthesis. | ||
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