Evaluation of the effectiveness of probiotic-based preparations against genetically characterize multi-drug resistant Escherichia coli- PV235949 causing urinary tract infections | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 21 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.428516.3275 | ||
| Authors | ||
| Amr G. kouto* 1; Wael Kasem1; Amal M. Soliman2; Amr Mohamed Shehabeldine1; Alsayed E. Mekky1 | ||
| 1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt | ||
| 2Medical Microbiology and Immunology Department, Faculty of Medicine Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| Background: Probiotics are live, healthy microorganisms that are present in fermented foods and milk. Urinary tract infections (UTIs) triggered by bacteria tolerant to three or more antibiotic classes are commonly referred to as multidrug resistant (MDR) bacteria. The fact that these "superbugs" have few available treatments makes them an important danger to the public. Methods: Probiotic bacteria were isolated from a milk sample. Additionally, 15 different antibiotics were used to investigate the sensitivity of pathogenic bacteria that were obtained from patient urine at Sayed Galal Hospital. By testing for intestinal and gastric tolerance as well as bile and acid tolerance, the bacterium's probiotic potential was verified. Molecular identification was used to identify the MDR and probiotic bacteria. GC-MS and HPLC were used to identify a number of bioactive components in the Lacticaseibacillus rhamnosus extract. The agar diffusion assay was used to evaluate the L. rhamnosus extract against MDR E. coli and determine its minimum inhibitory concentration (MIC). The L. rhamnosus extract's antioxidant levels were compared to an appropriate standard. The extract from L. rhamnosus was tested for cytotoxicity against Vero and PC-3 cells. Antiviral impact of L. rhamnosus extract was examined towards Adeno40 and COX-B4 Results: The number PV235949 was used to identify and isolate MDR E. coli from urine. However, a cod of PV235952.1 was used to identify L. rhamnosus that was isolated from cream. Its probiotic properties were demonstrated by L. rhamnosus's strong tolerance to pH, bile acid, and gastric and intestinal juice. L. rhamnosus extract included 13 different volatile chemicals, with the most common ones being D-fructose, diethyl mercaptal, pentaacetate, and E-9-tetradecenoic acid. The common phenolic chemicals were also ferulic acid and gallic acid. However, the most common flavonoids were apigenin and catechin. With a MIC of 62.5±1.6 µg/ml and a zone diameter of 22.13±1.2 mm, the L. rhamnosus extract demonstrated a promising anti-E. coli effect. With an IC50 of 23.54±2.2 µg/ml, the L. rhamnosus extract demonstrated good antioxidant properties. The extract from L. rhamnosus had a negligible effect on Vero cells (IC50 = 39.97± 0.38), and it showed a potential anticancer effect on PC-3 (IC50 = 18.24±0.41 µg/ml). Cox-B4 and Adeno-40 were significantly inhibited by L. rhamnosus extract, particularly when the extract was present at 31.25 µg/ml. Conclusion: A variety of bioactive compounds found in L. rhamnosus PV235952.1 may have played a crucial part in its antibacterial activity against MDR E. coli isolated from urine, anticancer, and antioxidant properties, as well as its remarkable safety for normal cells. Further investigations with animals are required to validate the physiological impacts of L. rhamnosus. | ||
| Keywords | ||
| Probiotic; antibacterial; antioxidant | ||
|
Statistics Article View: 5 |
||
