Isolation of Fusarium fujikuroi AUMC16188 from Alexandria, Egypt as a novel tannase producer exhibiting potent antioxidant activity | ||
| Archives of Agriculture Sciences Journal | ||
| Volume 8, Issue 2, August 2025, Pages 184-201 PDF (797.37 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/aasj.2025.460669 | ||
| Authors | ||
| H. H. H. El-Wahsh1; N. F. Abo-Dahab1; F. SH. El-Shenawy* 1; A. A. Amara2; Nawal Abd El-Baky2 | ||
| 1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt | ||
| 2Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box 21934, Alexandria, Egypt | ||
| Abstract | ||
| Tannase represents a technically crucial enzyme with significant potential to replace hazardous chemicals in various industrial applications. This study reports the successful isolation and screening of novel tannase-producing fungi from Egyptian soils. Among 68 fungal isolates obtained from 24 soil samples, Fusarium fujikuroi AUMC16188 emerged as the most promising strain, demonstrating exceptional tannase production (1399.73 U/mL; 264.1 U/mg) under optimized conditions. Genetic identification through ITS sequencing confirmed its novelty as a previously unreported tannase source. Remarkably, the purified enzyme exhibited superior antioxidant activity, showing an IC₅₀ of 1.18 µg/mL in 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays-approximately 6.5-fold more potent than Trolox. These findings establish Fusarium fujikuroi AUMC16188 as a dual-function biocatalyst with substantial biotechnological potential for sustainable industrial applications. | ||
| Keywords | ||
| Screening; isolation; application; soil fungi; Fusarium fujikuroi; tannase | ||
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