Hematological changes in Theba pisana induced by sublethal treatments of certain pesticides | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 14, Issue 2, December 2006, Pages 179-190 PDF (189.89 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.2006.460743 | ||
| Authors | ||
| Y. Abo-Bakr* 1; F. A. Kassem2; H. I. El-Deeb1; El A. M. Abdallah2 | ||
| 1Department of Agricultural Animal Pests, Plant Protection Research Institute, Agricultural Research Center | ||
| 2Pesticide Chemistry Dept. Faculty of Agriculture, Alexandria University. | ||
| Abstract | ||
| The sublethal effects of three pesticides (methomyl, chlorpyrifos and metaldehyde) on the hemolymph of land snail Theba pisana including number of hemocytes, hemocytic lysosomes as well as hemolymph free amino acids using wheat bran laboratory formulated toxic baits were investigated. The results revealed that hemocyte counts were increased significantly with all tested pesticides, except chlorpyrifos which did not appear any effect on the hemocyte counts. Maximum increase was observed after 6 hours of exposure, with time increase, the number of hemocytes decreased but still higher than control. Neutral red retention time assay revealed an effect of sublethal concentrations of tested pesticides on hemocytic lysosomal membrane. Retention time was reduced when concentrations of all tested pesticides increased. Free amino acids in the hemolymph were affected by pesticide treatments. There was no patterned profile of responses, but free amino acids sometimes increased and sometimes decreased with treatments. Aspartic acid concentration elevated when T. pisana was treated by methomyl or chlorpyrifos. Also, histidine and arginine concentrations elevated due to chlorpyrifos treatment. In contrary, cysteine concentration decreased as a result of methomyl and metaldehyde treatments. | ||
| Keywords | ||
| Land snail; pesticides; hemolymph; hemocytes; lysosomes; amino acids; sublethal effects | ||
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