Effect of on Alzheimer′s disease induced in rats | ||
| Journal of the Arab Society for Medical Research | ||
| Volume 8, Issue 1, January 2013, Pages 1-11 PDF (1.37 M) | ||
| Authors | ||
| Nemat A.Z. Yassin; Siham M.A. El-Shenawy; Karam A. Mahdy; Nadia A.M. Gouda; Abd El-Fattah H. Marrie; Abdel Razik H. Farrag; Bassant M.M. Ibrahim | ||
| Abstract | ||
| Background/aim Alzheimer's disease (AD) is a progressive neurodegenerative disorder. Increased oxidative stress has been shown to be a prominent and early feature in AD. Medicinal plants with antioxidant activities have been used traditionally in the treatment of several human diseases. The present study aimed to investigate the possible prophylactic and therapeutic effects of aqueous infusions of on AD induced in rats. Materials and methods Ninety adult male Sprague Dawley rats were enrolled in this study and were divided into 9 groups (ten each). Groups 1-5 for the protective study, 6-9 for the therapeutic study as follows: 1st group: negative control group in which rats were given daily oral dose of 1 ml tab water, 2nd group: induction of animal model mimicking AD by daily oral administration of aluminum chloride (AlCl3) to rats in a dose of 17 mg/kg for 4 successive weeks; 3rd, 4th, and 5th groups: rats were orally given rivastigmine (0.3 mg/kg/day), (45 and 90 mg/kg /day respectively), for two weeks followed by combination of each treatment with AlCl3 for another four successive weeks. Groups 6-9 for the therapeutic study: 6th group: AD induced group which acted as a model mimicking AD in humans received orally 1 ml of tab water only for 12 successive weeks and served as therapeutic untreated group. 7th, 8th and 9th groups: AD rats treated orally with rivastigmine (0.3 mg/kg/day), (45 and 90 mg/kg /day respectively) daily for 12 successive weeks. At baseline (before induction of AD), before treatment, then after each treatment, behavioral stress tests as activity cages, rotarod, and T-maze tests were done. At the end of all experiments rats' brains were dissected and divided sagitally into two portions, the first portion was homogenized for determination of acetylcholine (Ach) and acetycholinesterase (AchE) levels. The second portion was used for histopathologic examination. Results The present study indicated that when was used for treatment of AlCl3 induced AD, its high dose only produced increased activity of rats in the activity cage, duration of rats revolving on the rotarod and reduction in the duration taken by rats to reach food in the T-maze test. Both doses produced elevation of Ach level and reduction of AchE activity in brain homogenates. These results were consistent with the histopathological findings in brain tissues where, the neurons appear more or less like normal ones. Conclusion This study revealed that the treatment of AD-induced rats with aqueous infusions of significantly ameliorates the neurodegenerative characteristics of ADs in rats. | ||
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