Effect of spp., and isolated from polluted drainage water on rats | ||
| Journal of the Arab Society for Medical Research | ||
| Volume 11, Issue 1, January 2016, Pages 1-8 PDF (12.07 M) | ||
| DOI: 10.4103/1687-4293.186778 | ||
| Authors | ||
| Gamal A. Osman; Medhat M. Menshawy; Abdel Razik H. Farrag | ||
| Abstract | ||
| Background/aim Water pollution not only damages the environment but also kills wildlife. The present study aimed to investigate the effect of , and , which were isolated from drainage water at El-Rahawey area, Giza, Egypt, on experimental rats. Materials and methods Water samples were collected from the subsurface layer for bacteriological examination, stored in an ice box, and delivered to the laboratory and analyzed to count the total viable bacterial counts/ml using the most probable number technique. The isolated bacteria were given to rats for 21 days at a dose equal to 10/ml and the liver and kidney were dissected for histopathological and histochcemical studies. Results Results showed that the average counts at 22 and 37°C were 10 and 10CFU/ml, respectively, whereas the average counts by using the most probable number technique/100 ml were 10, 10, 10, 10, and 10 for total coliforms, fecal coliforms, , group, and total staphylococci, respectively. Isolation of pathogens from water sources were identified by using membrane filter technique on specific media. The histopathological examination of the liver of treated rats with , , and revealed swollen hepatocytes with decreased sinusoidal spaces and widely distributed necrotic foci. In the kidney, renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. The histochemical study indicated the depletion of staining of protein and polysaccharides. Conclusion , and that are isolated from polluted drainage water cause histological and histochemical changes in the liver and kidney of rats. | ||
| Keywords | ||
| Drainage water; Staphylococcus aureus; Escherichia coli; histochemical; Histopathological; Rats; Salmonella Typhi | ||
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