seed reduced galectin-3 level and liver fibrosis in thioacetamide-induced liver injury in rats | ||
| Journal of the Arab Society for Medical Research | ||
| Volume 12, Issue 2, July 2017, Pages 46-55 PDF (1.36 M) | ||
| DOI: 10.4103/jasmr.jasmr_8_17 | ||
| Authors | ||
| Ahmed M. Salem; Karam A. Mahdy; Nahla S. Hassan; Gamila S. M. El-Saeed; Abdel Razik H. Farrag; Mahmoud A. Abdel Monem | ||
| Abstract | ||
| Background/aim Fibrosis represents the final common pathway of chronic tissue injury. Galectin-3 (Gal-3) is an important regulator of fibrosis that links chronic inflammation to fibrogenesis. We investigated the effect of seed (black seed), a common hepatoprotective natural remedy, on Gal-3 level and progression of liver fibrosis in thioacetamide (TA)-induced liver injury in rats. Materials and methods Forty male Wistar rats were used in this study and were divided into four groups of 10 rats each. Group I served as control, groups from II to IV were intoxicated by TA (200 mg/kg body weight); meanwhile group III was treated with silymarin (50 mg/kg body weight) and group IV was treated with black seed (50 mg/kg body weight). Gal-3, transforming growth factor β1 (TGF-β1), some antioxidant and oxidative stress biomarkers were determined in the liver tissue homogenate. Moreover, serum liver function parameters, total cholesterol, triacylglycerols, and plasma glucose were determined. Quantitative measurement of fibrotic areas was achieved using computerized image analysis system. Results TA administration caused significant elevations in the levels of liver Gal-3, TGF-β1, malondialdehyde, nitric oxide and serum alanine transaminase and aspartate transaminase activities, total bilirubin, total cholesterol, triglycerides, and plasma glucose levels. Meanwhile, significant decreases were recorded in the level of liver total antioxidant capacity, catalase activity, and serum levels of total protein and albumin. Histopathological observation showed severe damage in the liver and presence of fibrotic areas. Treatment with silymarin and black seed resulted in decreasing levels of liver Gal-3 and TGF-β1 and marked improvement in liver functions, as well as reducing the fibrotic areas in the liver. Gal-3 exhibited positive correlation with TGF-β1, malondialdehyde, nitric oxide, alanine transaminase and aspartate transaminase, while it negatively correlated with total antioxidant capacity and catalase. Conclusion Black seed reduced liver Gal-3 level and ameliorated fibrogenesis in liver due to TA administration. | ||
| Keywords | ||
| Black seed; galectin-3; Liver fibrosis; Rats; Thioacetamide | ||
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