Anticancer, antioxidant, and antihyperlipidemic effects of royal jelly | ||
| Journal of the Arab Society for Medical Research | ||
| Volume 17, Issue 1, January 2022, Pages 68-76 PDF (1.48 M) | ||
| DOI: 10.4103/jasmr.jasmr_3_22 | ||
| Abstract | ||
| Background/aim Royal jelly (RJ) is a natural product obtained from honey bees and claimed to possess diverse health benefits. The aim of the present research was to search some of such health claims, including antioxidant, anticancer, and antihyperlipidemic effects, so as to support or negate such claims. Materials and methods RJ was tested for its antioxidant, anticancer, and antihyperlipidemic effects. The antioxidant effect was screened using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picryl-hydrazyl-hydrate, and ferric reducing antioxidant power assays. The anticancer effect was carried out by applying MTT assay using human cancer cell line from breast (MCF-7, breast adenocarcinoma) and from liver (Huh-7, hepatocellular carcinoma). The antihyperlipidemic effect was studied in a Triton X-100-induced hyperlipidemic rat model. The rats were divided into three groups; normal control, hyperlipidemic control, and hyperlipidemic group where rats were given 300 mg RJ/kg rat bodyweight as daily oral dose for 2 weeks before Triton injection and continued 3 days after the injection. Plasma triglycerides, total cholesterol (TC), high-densitylipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol, malondialdehyde, and the activities of transaminases (alanine transaminase and aspartate transaminase) were analyzed in all rats. The ratio of TC/HDL-C was calculated as a cardiovascular risk factor. Livers of all rats were investigated for any histopathological changes. Results demonstrated antioxidant activity with different degrees according to the assay type ranging from 0.43 to 5.634 ΜM Trolox eq/mg RJ. The anticancer effect showed IC50 of 51.133 and 107.332 Μg/ml from RJ toward MCF-7 and Huh-7, respectively. The animal experiment demonstrated significant reduction in the activities of alanine transaminase and aspartate transaminase, levels of malondialdehyde, triglycerides, TC, low-density lipoprotein-cholesterol with concomitant elevation in HDL-C, and a decrease in TC/HDL-C, with improvement of liver histopathology in the group given RJ compared with the hyperlipidemic control group. Conclusion Within the extreme of the present research, RJ was efficient as antihyperlipidemic and hepatoprotective agent and has mild to moderate antioxidant activity according to the screened assays together with anticancer potential in cell lines, which was superior against MCF-7 compared with Huh-7. | ||
|
Statistics Article View: 1 PDF Download: 1 |
||
