Preparation and in Vitro Antifungal Evaluation of Difenoconazole Nanoemulsion for Efficient Transport into Fungal Cells | ||
| Assiut Journal of Agricultural Sciences | ||
| Volume 56, Issue 4, October 2025, Pages 211-226 PDF (1.21 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajas.2025.393026.1497 | ||
| Authors | ||
| Islam K. F. Abd-Elghany; Entsar I. Rabea* ; Gehan I. Kh. Marei; Hoda M. Nasr; Moustafa A. Abbassy | ||
| Department of Plant Protection, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt. | ||
| Abstract | ||
| Recent advances in nanotechnology have paved the way for the development of nano-delivery systems. These systems enable the efficient delivery of fungicides to target pathogens, enhancing their bioavailability while minimising environmental and human health risks. A difenoconazole nanoemulsion was prepared using an ultrasonic method. The nanoemulsion (NE) is stable at room temperature, cold and heat storage, and many technical indicators meet the requirements. A transparent NE material with a narrow size distribution, suitable pH and viscosity was obtained. Scanning electron microscopy (SEM) analysis revealed the formation of discrete nanodroplets with sizes ranging from 66.67 to 91.53 nm. In vitro antifungal experiments were performed against three plantpathogenic fungi (i.e., A. Alternata, F. oxysporum and R. stolonifer), the difenoconazole nanoemulsion demonstrated high antifungal activity (EC50 = 112.93, 151.73 and 857.22 mg/L for A. alternata, F. oxysporum and R. stolonifer, respectively). Therefore, difenoconazole nanoemulsion showed high inhibition of spore germination (I (%) = 60.23 and 65.22 %, for A. alternata and F. oxysporum, respectively). The nanoemulsion has better permeability through fungal spore cells, giving better results compared to the commercially available difenoconazole emulsifiable concentrate. | ||
| Keywords | ||
| A. alternata; Antifungal activity; Difenoconazole nanoemulsion; F. oxysporum; R. stolonifer | ||
|
Statistics Article View: 4 PDF Download: 1 |
||
