Astaxanthin attenuates diabetic cardiomyopathy in rats by downregulation of HMGB1/TLR4/NF-kB signaling pathway | ||
| Medicine Updates | ||
| Articles in Press, Accepted Manuscript, Available Online from 22 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/muj.2025.427277.1268 | ||
| Authors | ||
| Soha M.Y. Mohamed1; Suzan A Khodir* 2; Noha Abd El-aziz3; Mohamed Ahmed Greash4; Marian F. L. Abdelmalak5; Mai M. Abdalraouf6; Rofanda M Bakeer7; Hidi A. A.Abdellatif8; Asmaa I Bayomi9; Amany Said Sallam10; Waleed Mahmoud Ragab11; Mona Mohamed Ahmed12; Safaa Mohamed abdel wahab13 | ||
| 1Medical Physiology Department, Faculty of Medicine Ain Shams University, Cairo, Egypt. Medical Physiology Department, Faculty of Dentistry, Misr International University, Cairo, Egypt | ||
| 2Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt. | ||
| 3Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt | ||
| 4Internal Medicine Department, Faculty of Medicine, Suez Canal University, Egypt. | ||
| 5Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt | ||
| 6Cardiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt. | ||
| 7Pathology Department, Galala University, Suez, Egypt, Pathology Department , Faculty of medicine Helwan University, Cairo, Egypt | ||
| 8Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University (SCU), Ismailia 41522, Egypt; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, King Salman International University, | ||
| 9Zoology department, Faculty of Science, Menoufia University. | ||
| 10Department of Pharmacology and Toxicology, Faculty of Pharmacy, Menoufia University, 32511 Shebin El-Kom, Menoufia, Egypt | ||
| 11Anatomy and Embryology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt, Anatomy and Embryology Department, Faculty of Medicine, Galala University, Attaka, Suez, Egypt | ||
| 12Department of physiology, Faculty of medicine, Cairo University, Cairo, Egypt, Biophysiology department, Ibn Sina National College for Medical Studies, Jeddah, Saudi Arabia | ||
| 13Medical Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt | ||
| Abstract | ||
| Abstract Background: Cardiomyocyte inflammation, oxidative stress, and myocardial metabolic disruption are among the pathologic abnormalities that can result from diabetic cardiomyopathy (DCM), an independent diabetic cardiac disease. Astaxanthin (ASX) possesses anti-inflammatory and antioxidant properties. Objective: to demonstrate the potential underlying mechanisms and cardioprotective impact of ASX in DCM. Material and methods: Thirty male rats were split into: DCM, DCM+ASX, and control (10/group). Evaluations of the following were conducted: tibial length/LVW, Serum cTnI, LDH, CK-MB, glycosylated Hb A1c, Cholesterol and Triglyceride, HOMA-IR index, and Cardiac MDA, SOD, TNF-α, IL-6, IL-10 and Cardiac gene expression of HMGB1, TLR4, and NF-kB. Additionally, the heart was examined histologically and immunohistochemically. Results: The DCM group's cardiac SOD and cardiac IL-10 were dramatically lower than control, but the measured LVW/tibial length, serum glucose, serum insulin, HOMA-IR index, serum glycosylated Hb A1c, serum cholesterol, serum triglyceride, serum cTnI, serum LDH, serum CK-MB, cardiac MDA, cardiac TNF-α, cardiac IL-6, and cardiac gene expression of HMGB1, TLR4, and NF-kB were all dramatically higher in the DCM than the control. The DCM group's TLR4 and NF-kB cardiac immunoreaction was dramatically lower than the control group's. ASX considerably reduced the alterations brought on by diabetic cardiomyopathy. Conclusion: ASX ameliorated diabetic cardiomyopathy by inhibiting the cardiac HMGB1/TLR4/NF-kB signaling cascade and demonstrating anti-inflammatory, antioxidant, hypoglycemic, and lipid-lowering qualities. | ||
| Keywords | ||
| Astaxanthin; Diabetic Cardiomyopathy; HMGB1; TLR4; NF-kB | ||
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