In vitro antagonistic activity of Trichoderma harzianum against Aspergiluus fumigatus | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 22 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.418993.3160 | ||
| Authors | ||
| Hayder Jabbar lrshayyid* ; Mohammed J. Hanawi* | ||
| Department of Biology, College of Science, Wasit University, Iraq | ||
| Abstract | ||
| Background and Aim: Aspergillosis in Aspergillus fumigatus is perilous to immunocompromised patients. This study evaluated the antifungal efficacy of Trichoderma harzianum as a bio control to A. fumigatus. Methods: The study was conducted from 1 September 2024 to 1 September 2025 in the Biology Department laboratory, College of Science, University of Waist. Trichoderma harzianum culture filtrates were prepared in potato dextrose broth and adjusted to final concentrations of 50%, 25%, and 10% (v/v). The antifungal activity against A. fumigatus was assessed using the poisoned food technique, disc diffusion, and dual culture methods. The percentage of growth inhibition was measured and analyzed statistically using ANOVA and LSD at p < 0.05. Results: The results revealed that the growth inhibition of Aspergillus fumigatus varied significantly according to the concentration of T. harzianum culture filtrates. All tested concentrations showed a significant inhibitory effect compared with the control. The highest inhibition was observed at 50% concentration (73.2%), followed by 25% (47.14%), and 10% (20.66%). In the dual culture assay, T. harzianum exhibited strong inhibitory activity, completely overgrowing the pathogen and covering the entire surface of A. fumigatus. Conclusion: The fungus Trichoderma harzianum recorded high inhibitory activity and completely overgrew the test pathogen A fumigatus and grow on the entire surface of the tested pathogen A fumigatus. | ||
| Keywords | ||
| Biocontrol; Trichoderma Aspergillus species; Dualculture; Mycoparasitism | ||
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