Evaluation of renal dialysis on biofilm production profiles of pathogenic bacteria isolated from patients attending the dialysis unit in Ramadi City | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 22 October 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.425837.3244 | ||
| Authors | ||
| Wassan Shaker Ajami1; Haidar Kadum Yakob* 2 | ||
| 1Department of Biology, College of Education for Pure Sciences, University Of Anbar, Iraq | ||
| 2Department of Biology, College of Education for Pure Sciences, University of Anbar, Iraq | ||
| Abstract | ||
| Background: Bacterial infection is one of the most prominent challenges facing patients with kidney failure who undergo dialysis, as bacteria have many mechanisms that make them resistant, and among these mechanisms is the ability of some species to form biofilms that contribute to their stability on vital and abiotic surfaces, and enhance their viability and reproduction. This research aims to isolate and diagnose pathological bacteria from urine samples of patients in Dialysis unit in the city of Ramadi, and investigate its ability to produce biofilms before and after dialysis treatment sessions. Methods: Urine samples [160] were collected from 80 patients [two samples from each patient before and after dialysis]. The samples were cultured on differential culture media, diagnosed based on culture characteristics, biochemical tests and VITEK 2 compact. The ability of bacterial isolates to form biofilms was tested using micro-titer plate method by using violet crystal dye. Results: The results showed that the Gram-negative bacteria were the most common, especially Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae, these species have shown a high ability to produce biofilms, with the percentage of isolates produced before dialysis treatment 91.25%, and after dialysis treatment 86.25%. The study also showed that patients aged 51-70 years, who have been infected for more than a year, were the most representative among the isolates produced. Conclusion: The findings from this study showed that biofilm-producing bacteria represent a real biological hazard in dialysis units, and require careful interventions at the level of early diagnosis, in addition to reviewing sterilization and clinical care protocols in these units, to ensure reduce infection rates and improve patients' quality of life. | ||
| Keywords | ||
| Antibiotic susceptibility; Biofilm production; Dialysis patients; Pathogenic bacteria; Escherichia coli | ||
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